通痹方热奄包外敷对家兔退变颈椎间盘细胞凋亡相关基因Caspase-3和Bcl-2 mRNA的影响  

The Effect of the External Application of Tongbi Hot Compress on mRNA Expression Levels of Caspase-3 and Bcl-2 in Rabbit Degenerated Intervertebral Discs

作　　者：徐银琴 王光义[1] XU Yinqin;WANG Guangyi(Department of Accupuncture,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学附院针灸科,贵州贵阳550004

出　　处：《贵州医科大学学报》2020年第4期438-443,共6页Journal of Guizhou Medical University

基　　金：贵州省中医药局中医药、民族医药科学技术研究课题(QZXY-2016-075);贵阳市科技计划项目[筑科合同(2019)9-1-4]。

摘　　要：目的:探讨通痹方热奄包外敷对家兔退变椎间盘细胞凋亡相关基因天冬氨酸特异性蛋白酶-3(Caspase-3)和B淋巴细胞瘤-2(Bcl-2)mRNA表达的影响。方法:普通级新西兰大白兔18只随机均分为空白组(正常兔,不做任何干预)、模型组(颈椎病模型兔,不予任何治疗)及热敷组(颈椎病模型兔,通痹方热奄包外敷治疗),各组家兔于造模前、造模第30天及治疗结束后拍摄颈椎X线并评分;治疗15次后留取各组家兔C3-4和C4-5椎间盘,采用苏木精-伊红(HE)染色观察C3-4椎间盘组织形态学变化并评分,实时荧光定量法检测C4-5椎间盘Caspase-3和Bcl-2 mRNA相对表达量。结果:模型组和热敷组家兔造模后颈椎X线评分分别较空白组升高,热敷组家兔治疗后颈椎X线积分改善程度较模型组大,差异均有统计学意义(P<0.05);HE染色结果显示,模型组家兔C3-4椎间盘评分比空白组升高,热敷组评分比模型组降低,差异均有统计学意义(P<0.05);与空白组相比,模型组家兔C4-5椎间盘内Casepase-3 mRNA表达升高、Bcl-2 mRNA表达降低,差异均有统计学意义(P<0.05);与模型组相比,热敷组家兔椎间盘内Casepase-3 mRNA表达降低,Bcl-2 mRNA表达升高,差异均有统计学意义(P<0.05)。结论:通痹方热敷可通过上调Bcl-2 mRNA表达、抑制Casepase-3 mRNA表达减少椎间盘细胞凋亡,延缓椎间盘退变。Objective:To investigate the effect of the external application of tongbi hot compress on mRNA expression levels of Caspase-3 and Bcl-2 in rabbit degenerated intervertebral discs.Methods:Eighteen ordinary New Zealand white rabbits were randomly divided into a blank group(normal rabbits),a model group(rabbits with cervical spondylosis)and a hot compress group(rabbit with cervical spondylosis+external application of tongbi hot compress).The rabbits were photographed and scored by cervical X-ray before the model,on the 30th day of the model and after the treatment.After 15 treatments,the C3-4 intervertebral discs were stained with hematoxylin-eosin(HE)to observe and score the morphological changes of intervertebral discs,and real-time qPCR was used to detect the relative expression of Caspase-3 and Bcl-2 mRNA in C4-5 intervertebral discs.Results:The X-ray scores were higher in the model group and the hot compress group than those in the blank group after modeling,and the improvement of X-ray scores was higher in hot compress group than that in model group(P<0.05).HE staining showed that the C3-4 intervertebral disc scores were higher in model group than those in blank group,while the scores were lower in hot compress group than those in model group(P<0.05).When compared with the blank group,Casepase-3 mRNA levels were increased,while Bcl-2 mRNA levels were decreased in the model group(P<0.05);when compared with the model group,Casepase-3 mRNA levels were decreased,while Bcl-2 mRNA levels were increased in hot compress group(P<0.05).Conclusion:Tongbi hot compress can reduce the apoptosis of intervertebral disc cells and delay the degeneration of intervertebral disc by up-regulating the Bcl-2 mRNA and downregulating Casepase-3 mRNA.

关 键 词：椎间盘 细胞凋亡 通痹方 热敷 天冬氨酸特异性蛋白酶-3 B淋巴细胞瘤-2 

分 类 号：R244.9[医药卫生—针灸推拿学]