新肝宝对鲶爱德华氏菌感染黄颡鱼的保护作用研究  

Study on the Protective Effect of Xin Gan Bao on Yellow Catfish Pelteobagrus fulvidraco Infected with Edwardsiella ictaluri

作　　者：喻运珍 余少梅[2] 袁改玲[3] 邓平[1] 张立强 罗杨志 胡少迪[2] 丁桂珍 程颖红 YU Yun-zhen;YU Shao-mei;YUAN Gai-ling;DENG Ping;ZHANG Li-qiang;LUO Yang-zhi;HU Shao-di;DING Gui-zhen;CHENG Ying-hong(Wuhan Academy of Agriculture Science&Technology,Wuhan 430207,China;Wuhan Chopper Fishery Bio-Tech Co.,Ltd.,Wuhan 430207,China;Huazhong Agricultural University,Wuhan 430070,China.)

机构地区：[1]武汉市农业科学院,武汉430207 [2]武汉中博水产生物技术有限公司,武汉430207 [3]华中农业大学,武汉430070

出　　处：《中国兽药杂志》2020年第4期56-62,共7页Chinese Journal of Veterinary Drug

基　　金：现代农业产业技术体系专项资金资助“CARS-46”;湖北省科技计划项目(2018BEC482)。

摘　　要：为了考察新肝宝对鲶爱德华氏菌感染黄颡鱼的保护作用,采用不同粉碎粒度的新肝宝拌饲投喂7 d,随后用鲶爱德华氏菌腹腔注射感染,观察死亡率、酶学指标及组织病理变化。结果显示,新肝宝组死亡率(48.57%和44.28%)低于阴性对照组(54.29%),溶菌酶及超氧化物歧化酶活性显著高于对照组(P<0.05),肝脏、肾脏、脾脏损伤情况均小于阴性对照组,其中600目作用效果优于200目。结果表明,新肝宝能够提高黄颡鱼非特异性免疫力,增强黄颡鱼对鲶爱德华氏菌的抵抗能力,在一定程度上减轻鲶爱德华氏菌感染导致的肝、脾、肾损伤,其中600目作用效果优于200目。The study was aimed to evaluate the protective effect of Xin Gan Bao on yellow catfish(Pelteobagrus fulvidraco)infected with Edwardsiella ictaluri.The fish were intraperitoneally injected with Edwardsiella ictaluri after feeded with diet containing Xin Gan Bao of 200-mesh and 600-mesh for 7 d.Then the mortality,enzyme activities and histopathology changes were investigated.The results showed that mortalities of Xin Gan Bao groups(48.57%and 44.28%)were lower than that of the negative control group(54.29%).The levels of lysozyme(LZM)and superoxide dismutase(SOD)were higher than that of the control group significantly(P<0.05).The damage of liver,kidney and spleen were lighter than the negative control group.And Xin Gan Bao of 600-mesh had better effect than the 200-mesh.It indicated that Xin Gan Bao could improve the non-specific immunity and resistance to Edwardsiella ictaluri of yellow catfish,and reduce the damage caused by Edwardsiella ictaluri infection.And Xin Gan Bao of 600-mesh had better effect than the 200-mesh.

关 键 词：新肝宝 黄颡鱼 鲶爱德华氏菌 

分 类 号：S853.7[农业科学—临床兽医学]