外源性雌孕激素联合给药诱导雌性大鼠人工动情周期的方法学探讨  

Methodological Study on Induction of Artificial Estrous Cycle in Female Rats by Exogenous Estrogen and Progesterone Combined A dministration

作　　者：刘坤[1] 王玉杰[1] 李亚琼 张浩[1] 张胜男[1] 魏盛[1] Liu Kun;Wang Yujie;Li Yaqiong(Animal Beharior Experimental Platform of Shandong University of Traditional Chinese Medicine,Shandong 250355,China)

机构地区：[1]山东中医药大学动物行为学实验平台,济南250355

出　　处：《医学研究杂志》2020年第3期61-64,69,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81703941);山东省中医药科技发展计划项目(2017-005);山东中医药大学SRT项目(2018097)。

摘　　要：目的对Wistar雌性性成熟大鼠在卵巢切除术后进行外源性雌、孕激素注射以诱导人工动情周期,为诱导大鼠同步且规律的动情周期提供一种可靠的方法.方法用阴道电阻仪测量Wistar雌性大鼠阴道电阻值,按照动情周期是否规律将大鼠分为两组,对两组大鼠均进行卵巢切除术,恢复1周后进行雌孕激素联合给药法诱导大鼠的动情周期(动情周期规律大鼠作为对照),测量大鼠阴道电阻值,观察电阻值的变化趋势,判断其动情周期的规律性和同步性.结果对筛选出的10只动情周期规律的大鼠及90只动情周期不规律的大鼠均进行卵巢摘除术后的外源性雌孕激素联合给药诱导,大鼠的阴道电阻值显示诱导后的人工动情周期均呈现以4天为1个周期,持续两个周期的特点.结论外源性雌孕激素联合给药法可以诱导大鼠规律且同步的动情周期.Objective To provide a reliable method for inducing synchronous and regular estrous cycle in Wistar female mature rats by injecting exogenous estrogen and progesterone after ovariectomy.Methods V aginal resistanee of Wistar female rats was measured by vaginal resistance meter.According to whether the estrous cycle was regular or not,the rats were divided into two groups.Ovariectomy was performed in both groups,and estrogen and progesterone combined administration was used to induce the estrous cycle of rats one week later(estrous cycle regular rats as control).Vaginal resistance of rats was measured to observe the change trend of resistance value,and to judge the regularity and synchronization of estrous cycle.Results Ten rats with regular estrous cycle and 90 rats with irregular estrous cycle were induced by combined administration of exogenous estrogen and progesterone after ovariectomy.The vaginal resistance values of rats showed that the induced artificial estrous cycle was characterized by a cycle of 4 days and lasted for two cycles.Conclusion Exogenous estrogen combined with progesterone can induce regular and synchronous estrous cycle in rats.

关 键 词：Wistar雌性大鼠 激素诱导 动情周期 阴道电阻法 

分 类 号：R3[医药卫生—基础医学]