Conjugated polymer nanoparticles as fluorescence switch for selective cell imaging  

作　　者：Ke Peng Fengting Lv Huan Lu Jianwu Wang Hao Zhao Libing Liu Shu Wang 

机构地区：[1]Beijing National Laboratory for Molecular Sciences,Key Laboratory of Organic Solids,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China [2]College of Chemistry,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Chinese Chemical Letters》2020年第3期755-758,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.91527306,21661132006);the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16020804);the Youth Innovation Promotion Association CAS(No.2016029)。

摘　　要：Fluorescence switch plays a vital role in bioelectronics and bioimaging.Herein,we presented a new kind of facile electrostatic complex nanoparticles(ECNs)for fluorescence switching in cells and marking of individual cell.The ECNs were prepared by mixing positively charged poly(6-(2-(thiophen-3-yl)ethoxy)hexyl trimethylammonium bromide)(PT)and negatively charged diarylethene sodium salt(DAECOONa).DAE-COONa is a photoswitchable molecule which can be transformed between the ring-closed fo rm and ring-open form under the irradiation of UV or visible light.The closed-form of DAE-COONa can efficie ntly quench the fluorescence of PT through intermolecular energy transfer,while the open form of DAE-COONa does not influence the emission of PT.Thus,the fluorescence of ECNs can be modulated by light irradiation,and the ECNs with good fluorescence switching performance have been employed for fluorescence imaging and individual cell lighting up process successfully.We demonstrate that the electrostatic complex strategy provides a facile method to construct fluorescence switch fo r selective cell marking and imaging applications.

关 键 词：ELECTROSTATIC complex NANOPARTICLES CONJUGATED polymers Fluorescence switch CELL imaging SELECTIVE CELL LIGHTING up 

分 类 号：TB383.1[一般工业技术—材料科学与工程] Q2-33[生物学—细胞生物学]