1株NADC30-like重组猪繁殖与呼吸综合征病毒的分离鉴定及遗传变异分析  

ISOLATION,IDENTIFICATION AND GENETIC VARIATION OF A NADC30-LIKE STRAIN SDWH OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS

作　　者：邓紫艳 张锋 董雅琴[1] 吴发兴[1] 段纲 李晓成[1] DENG Zi-yan;ZHANG Feng;DONG Ya-qin;WU Fa-xing;DUAN Gang;LI Xiao-cheng(China Animal Health and Epidemiology Center,Qingdao 266032,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]中国动物卫生与流行病学中心,青岛266032 [2]云南农业大学动物医学院,昆明650201

出　　处：《中国动物传染病学报》2020年第3期21-28,共8页Chinese Journal of Animal Infectious Diseases

基　　金：科技基础性工作专项(2012FY111000)。

摘　　要：为研究山东地区猪繁殖与呼吸综合征病毒(PRRSV)流行病毒株的遗传变异情况,本研究从山东省威海市某疑似猪繁殖与呼吸综合征(PRRS)发病猪场采集的猪肺脏组织中分离到1株PRRSV,命名为SDwh,并对其进行了全基因组序列测定、遗传演化分析和重组分析.结果显示:该病毒株在Marc-145细胞上生长良好,可见明显细胞病变;全基因组演化分析和同源性比对分析结果显示,SDwh株与美国病毒株NADC30和中国的NADC30-like位于同一分支;与北美病毒株NADC30的同源性最高,为93.1%;与我国分离的NADC30-like病毒株CHsx1401、JL580的同源性分别为91.1%和88.4%;与北美经典病毒株VR2332、中国HP-PRRSV JXA1和HuN4的同源性均为85.3%;与欧洲型病毒株Lelystad-virus(LV)同源性最低,为60.6%.与VR2332相比,Nsp2氨基酸序列比对结果显示,SDwh株存在NADC30-like病毒株典型的131个不连续氨基酸的缺失,同时在584~585位缺失2个氨基酸;GP5氨基酸序列比对结果显示,SDwh在GP5抗原表位上有氨基酸的突变.全基因组重组分析结果显示,SDwh株是一株重组病毒株,为NADC30与JXA1-P80的重组病毒,潜在的重组位点位于Nsp2中(2066 nt).本研究结果为PRRSV流行株的重组、演化分析和防控提供借鉴意义.To understand the genetic variation of Porcine reproductive and respiratory syndrome virus(PRRSV),a PRRSV strain SDwh was isolated in Marc-145 cells from the lung of a pig suspected PRRSV infected in Weihai of Shandong province and its complete genome sequence was amplified by PCR for genetic evolution and recombination analysis.The strain SDwh developed cytopathic effect(CPE)in Marc-145 cells.Phylogenetic analysis showed that the strain SDwh belonged to the same cluster with the NADC30 strain from American and NADC30-like strains from China.In addition,the SDwh had nucleotide homology of 93.1% with the American strain NADC30 and 91.1% and 88.4% with the Chinese NADC30-like strains CHsx1401and JL580.The nucleotide homology of the isolate SDwh was 85.3% with the American classical strain VR2332 and Chinese HP-PRRSV strains JXA1 and HuN4.As expected,the isolate SDwh had the low nucleotide homology of 60.6% with LV strain.The alignment of deduced amino acid sequences revealed that the isolate SDwh had 131 discontinuous amino acid deletion typical for NADC30-like strains and 2 amino acid deletion at 584-585 positions in Nsp2 and amino acid mutations in GP5 as compared with VR2332.These results suggested that the isolate SDwh was a recombinant strain between NADC30 and JXA1-P80 and the potential recombination site was located in Nsp2(2066 nt).This study provided additional information on recombination and evolution of PRRSV in Chinese pig population.

关 键 词：猪繁殖与呼吸综合征病毒 分离鉴定 重组 演化分析 

分 类 号：S852.659.6[农业科学—基础兽医学]