鸡可食性组织中尼卡巴嗪残留的测定高效液相色谱法  

DETERMINATION OF NICARBAZIN RESIDUE IN CHICKEN EDIBLE TISSUES USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：蒋永嘉 肖霞[1,2] 蓝玮璇 江礼捷 谢恺舟 卜仕金[3] 王志强 JIANG Yong-jia;XIAO Xia;LAN Wei-xuan;JIANG Li-jie;XIE Kai-zhou;BU Shi-jin;WANG Zhi-qiang(Laboratory of Veterinary Pharmacology and Toxicology,College of Veterinary Medicine,Hangzhou University,Hangzhou 225009,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Hangzhou 225009,China;College of Animal Science and Technology,Hangzhou University,Hangzhou 225009,China)

机构地区：[1]扬州大学兽医学院兽医药理毒理教研室,扬州225009 [2]江苏高校动物重要疫病与人兽共患病防控协同创新中心,扬州225009 [3]扬州大学动物科学与技术学院,扬州225009

出　　处：《中国动物传染病学报》2020年第3期73-79,共7页Chinese Journal of Animal Infectious Diseases

基　　金：修订《鸡可食组织中尼卡巴嗪残留量的测定高效液相色谱法》标准;现代农业产业技术体系专项资金;江苏高校优势学科建设工程资助项目(PAPD)。

摘　　要：为建立鸡可食性组织(皮脂、肌肉、肝脏、肾脏)中尼卡巴嗪的高效液相色谱(HPLC)检测方法,本研究采用乙腈提取组织样品中尼卡巴嗪,正己烷除脂;流动相为乙腈:超纯水(55:45,v/v);紫外检测波长347 nm.结果显示:各组织中尼卡巴嗪浓度在50~1000μg/kg范围内呈良好线性关系(R^2≥0.9992);各组织中检测限和定量限均分别为10μg/kg和50μg/kg;组织中尼卡巴嗪添加浓度为50、200、400μg/kg时,皮脂中药物平均回收率为(87.18±3.85)%,肌肉中药物平均回收率为(93.33±3.76)%,肝脏中药物平均回收率为(80.77±3.63)%,肾脏中药物平均回收率为(85.27±6.58)%;日内和日间变异系数分别小于4.20%和4.30%.该方法前处理简单省时,无需使用固相萃取,节约了试验成本与时间,为尼卡巴嗪的检测提供了方法学基础.In the present study,a simple,fast and reliable high performance liquid chromatography(HPLC)method was developed for determination of nicarbazin residue in chicken edible tissues,including fat,skins,livers,kidneys and muscles.Acetonitrile was used for extraction of Nicarbazin and hexane for removal of fat from chicken tissues.The mobile phase consisted of acetonitrile and ultrapure water with proportion of 55:45(v/v).The target compound was separated on a C18 column and detected by UV detection at 347 nm.The residue marker of Nicarbazin(DNC)in different chicken edible tissues showed good linear relationship ranging from 50-1000μg/kg(R^2≥0.9992).The limit of detection(LOD)and the limit of quantitation(LOQ)was 10 and 50μg/kg,respectively.When DNC was added to tissues,the average recovery at spiked DNC concentrations of 50,200,400μg/kg was(87.18%±3.85)in skins/fat,(93.33%±3.76)in muscles,(80.77%±3.63)in livers,and(85.27%±6.58)in kidneys.The intra-day and inter-day coefficients of variation in all the tissues at spiked concentration of 50,200,400μg/kg were less than 4.20% and 4.30%,respectively.The treatment procedure for chicken edible tissue samples was simple and cost efficient,which provided methodology for Nicarbazin determination.

关 键 词：鸡 尼卡巴嗪 HPLC 可食性组织 

分 类 号：S859.795[农业科学—临床兽医学]