不同水平切应力对早期内皮祖细胞功能的影响  

Effects of different levels of shear stress on the function of early endothelial progenitor cells

作　　者：曾丽金[1] 吴敬国[1] 唐皓[1] 黄振华[1] 刘志豪[1] 杨震[1,2,3] ZENG Li?jin;WU Jing?guo;TANG Hao;HUANG Zhen?hua;LIU Zhi?hao;YANG Zhen(Emergency department of the First Affiliated Hospital of Sun Yat sen University,510080;Department of Cardiology,the First Affiliated Hospital of Sun Yat sen University;Key Laboratory of auxiliary circulation of the Ministry of health)

机构地区：[1]中山大学附属第一医院急诊科、普内科,510080 [2]中山大学附属第一医院心内科 [3]中山大学附属第一医院卫生部辅助循环重点实验室

出　　处：《岭南急诊医学杂志》2020年第1期34-35,47,共3页Lingnan Journal of Emergency Medicine

基　　金：国家自然科学基金资助项目(81670220);广州市科技计划项目(201803010008);广州经济技术开发区国际科技合作项目(2017GH13)。

摘　　要：目的:本研究旨在观察切应力对早期内皮祖细胞体外功能的影响,以探讨切应力介导细胞修复血管损伤的可能性。方法:分离培养及鉴定外周血内皮祖细胞,予以10 dyn/cm^2和20 dyn/cm^2处理15小时,并观察不同水平切应力对内皮祖细胞体外功能的影响。结果:切应力处理后,内皮祖细胞的迁移、黏附和增殖功能明显增加,并且这种作用呈剂量依赖性。结论:10 dyn/cm2和20 dyn/cm2切应力显著增加内皮祖细胞体外功能和内皮修复能力,表明切应力介导的细胞疗法是修复血管内皮损伤的有效策略之一。Objective:To observe the effect of shear stress on the function of early endothelial progenitor cells(EPCs)in vitro,and to explore the possibility of shear stress?mediated cell repair of vascular injury.Methods:EPCs were isolated,cultured and identified.They were treated shear stress with 10 dyn/cm^2 and 20 dyn/cm^2 for 15 hours.Results:After shear stress treatment,the migration,adhesion and proliferation of EPCs increased significantly,and the effect was dose?dependent.Conclusion:Shear stress with 10 dyn/cm^2 and 20 dyn/cm^2 can significantly increase the function of EPCs in vitro and the ability of endothelial repair.

关 键 词：内皮祖细胞 切应力 血管损伤 功能 

分 类 号：R54[医药卫生—心血管疾病]