日本血吸虫感染小鼠外周血CD4^+T细胞亚群动态变化的研究  

Dynamics of CD4^+T cell subsets in peripheral blood of mice infected with Schistosoma japonicum

作　　者：王小溪 雷南行 潘卫庆 WANG Xiaoxi;LEI Nanxing;PAN Weiqing(Department of Tropical Medicine,Naval Military Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学热带病学教研室,上海200433

出　　处：《中国热带医学》2020年第4期295-300,共6页China Tropical Medicine

基　　金：国家自然科学基金(No.81972985)。

摘　　要：目的了解日本血吸虫感染小鼠外周血CD4^+T细胞亚群水平的动态变化情况,探讨感染进程中CD4^+T细胞亚群的免疫调控作用。方法通过日本血吸虫尾蚴腹贴法建立日本血吸虫感染小鼠模型,将18只6周龄C57BL/6J雄性小鼠随机分成正常对照组和日本血吸虫感染组,每组9只。通过流式细胞染色计数的方法检测感染3周、5周及8周后小鼠外周血CD4^+T细胞亚群中Th1、Th2、Th17、Treg细胞变化情况。结果随着日本血吸虫感染进程,与正常对照小鼠相比,感染小鼠外周血CD4^+T细胞亚群中Th1、Th2、Th17、Treg细胞比例均显著增加。不同细胞亚群比例增加的趋势不尽相同,其中Th1细胞在感染第3周占比较高但其增长较为缓慢,而感染前3周Th2、Treg细胞占比较低但其增加幅度较大,在第8周达到峰值;Th17细胞比例在感染前3周基本无变化,随后显著增加。结论日本血吸虫感染引起宿主CD4^+T细胞亚群比例发生变化,本研究发现感染显著增加小鼠外周血Th1、Th2、Th17、Treg细胞比例,感染后3周内小鼠外周血CD4^+T细胞亚群以Th1细胞为主,5周后Th2、Th17、Treg细胞逐渐占主导地位。本结果为研究日本血吸虫病免疫病理机制和特征提供基础。Objective To evaluate the dynamics of CD4^+T cell subsets in peripheral blood of mice infected with Schistosoma japonicum,and to investigate the immune regulatory function of CD4^+T cell subsets during infection.Methods After mice were infected with cercaria of Schistosoma japonicum by applying to abdomen walls,eighteen male C57BL/6J mice at 6 weeks of age were divided into the control group and infection group randomly(nine mice each group).The dynamics of CD4^+T cell subsets,such as Th1,Th2,Th17 and Treg cells,in peripheral blood of these mice were detected at 3,5 and 8 weeks post infection using a fluorescence-activated cell sorter(FACS).Results As the schistosome infection progress,the number of the CD4^+T cell subsets in peripheral blood of the infected mice is significantly increased in various trends,among which the Th1 cells account for a higher proportion at 3 weeks post infection and is increased slowly,while both Th2 and Treg cells account for a lower proportion at 3 weeks post infection,after which Th2 and Treg cells are highly elevated and peaked at 8 weeks post infection;There is no change of Th17 cells during the first 3 weeks after infection but the percentage of Th17 cells is significantly increased at 5 weeks after infection.Conclusion The schistosoma infection significantly increased the percentages of Th1,Th2,Th17 and Treg cells in peripheral blood of mice,among which the Th1 cells took the major part within 3 weeks after infection,and Th2,Th17 and Treg cells gradually dominated after 5 weeks during infection process.Our data provides a basis for the study of Schistosomiasis immunopathological mechanisms and features.

关 键 词：日本血吸虫 外周血 CD4^+T细胞亚群 

分 类 号：R383.24[医药卫生—医学寄生虫学]