伯氏疟原虫感染小鼠巨噬细胞、树突状细胞及其表面分子的变化  

Changes of macrophages,dendritic cells and their surface molecules in mice infected with Plasmodium berghei

作　　者：韩孟伊 陈冰霞 张杰森 张梦欣 李勇森 金晨曦 齐艳伟 HAN Mengyi;CHEN Bingxia;ZHANG Jiesen;ZHANG Mengxin;LI Yongsen;JIN Chenxi;QI Yanwei(The Third Clinical School,Guangzhou Medical University,Guangzhou,Guangdong 511436,China;Department of Pathogenic Biology and Immunology,Guangzhou Medical University,Guangzhou,Guangdong 511436,China)

机构地区：[1]广州医科大学第三临床学院,广东广州511436 [2]广州医科大学病原生物学与免疫学教研室,广东广州511436

出　　处：《中国热带医学》2020年第4期330-334,342,共6页China Tropical Medicine

基　　金：国家自然科学基金项目(No.81902087);广东省自然科学基金项目(No.2017A030310535);广东省大学生创新创业训练计划项目(No.S201910570023);广东省大学生创新创业训练计划项目(No.S201910570027);广州医科大学大学生创新创业训练项目(No.2018A036);广州医科大学第三临床学院大学生科研项目(No.2018A0024);广州医科大学第三临床学院大学生科研项目(No.2018A0020)。

摘　　要：目的探讨C57BL/6小鼠感染伯氏疟原虫ANKA株后脾脏不同免疫细胞的含量及其表达的细胞表面分子的变化。方法将10只C57BL/6小鼠分为正常对照组和感染组,每组5只,分别经尾静脉注射生理盐水和伯氏疟原虫虫株,6天后分离小鼠脾脏,制备单细胞悬液。通过流式细胞术检测小鼠巨噬细胞(Mφ)和树突状细胞(DC)及其细胞表面分子CD69、MHCⅡ、CD80、TLR2的百分比含量。结果感染组小鼠脾脏巨噬细胞所占比例低于正常组,差异无统计学意义(P>0.05);其表面分子CD69、CD80、MHCⅡ的表达水平较正常组均升高,且CD69、CD80的表达差异有统计学意义(P<0.01),而MHCⅡ的表达差异无统计学意义(P>0.05);其表达的TLR2百分比较正常组降低,差异无统计学意义(P>0.05)。感染组小鼠DC所占比例低于正常组,差异有统计学意义(P<0.05);感染组小鼠脾脏DC表达CD69、CD80水平较正常组均显著升高,差异有统计学意义(P<0.01);其表达TLR2、MHCⅡ所占比例均低于正常组,差异无统计学意义(P>0.05)。结论感染伯氏疟原虫的C57BL/6小鼠脾脏中DC含量降低,巨噬细胞和DC表达细胞因子CD69、CD80的能力上升,由此笔者认为巨噬细胞和DC可能会通过增加其自身CD69和CD80的表达水平来抵抗伯氏疟原虫的感染。Objective To investigate the changes of the contents of different immune cells and the expression of cell surface molecules in the spleen of C57BL/6 mice infected with Plasmodium berghei ANKA.Methods The number of 10 C57BL/6 mice were divided into the normal group and the infected group with 5 in each group,and normal saline and Plasmodium berghei were injected into the tail vein separately.Six days later,the spleens of all mice were isolated to prepare a single cell suspension.The percentage of macrophages and DCs and their cell surface molecules CD69,MHCⅡ,CD80 and TLR2 from the mice were detected by flow cytometry.Results The proportion of spleen macrophages in the infected group was lower than that in the normal group without significant difference(P>0.05),the expression of the surface factor CD69 and CD80 of spleen macrophages in the infected group were significantly higher than those in the normal group(P<0.01),the surface molecule MHCⅡof it was also higher than that of the normal group,but there was no significance(P>0.05),and the percentage of TLR2 was lower than that of the normal group,and there was no significance(P>0.05).The proportion of DC in the infected group was statistically lower than that in the normal group(P<0.05),the expression of the CD69 and CD80 of spleen DC cells in the infected group were significantly higher than those in the normal group(P<0.01),the proportion of surface molecule TLR2 and MHCⅡwas lower than those in the normal group,but the differences were not statistically significant(P>0.05).Conclusion The content of DC in the spleen of C57BL/6 mice infected with Plasmodium berghei decreased,and the proportion of the cytokines CD69 and CD80 expressed by macrophages and DCs increased.The authors believe that macrophages and DCs mainly resist the infection of Plasmodium berghei by increasing the expression of CD69 and CD80.

关 键 词：伯氏疟原虫 脾脏 免疫细胞 细胞表面分子 

分 类 号：R382.31[医药卫生—医学寄生虫学]