四溴双酚A对人体呼吸系统细胞16HBE和Beas2B的接触暴露毒性机制  

Toxicity mechanism of tetrabromobisphenol A to human respiratory system cells 16HBE and Beas2B

作　　者：宁美霖 胡俊杰 卢莉蓉 蔡仪威 李桂英[1] 安太成[1] Meilin Ning;Junjie Hu;Lirong Lu;Yiwei Cai;Guiying Li;Taicheng An(Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control,School of Environmental Science and Engineering,Institute o f Environmental Health and Pollution Control,Guangdong University of Technology,Guangzhou 510006,China)

机构地区：[1]广东工业大学环境健康与污染控制研究院,环境科学与工程学院,广东省环境催化与健康风险控制重点实验室,广州510006

出　　处：《科学通报》2020年第10期931-939,共9页Chinese Science Bulletin

基　　金：国家自然科学基金(41877363,41731279);国家杰出青年科学基金(41425015);广东省珠江人才计划本土创新团队项目(2017BT01Z032)资助。

摘　　要：四溴双酚A(TBBPA)是一种应用量非常大的化学品,广泛用在印刷电路板以及其他聚合材料的阻燃剂中.作为一种环境污染物,其普遍存在于各种非生物和生物介质中.本研究采用人体支气管细胞Beas2B和肺上皮细胞16HBE作为研究对象,开展了TBBPA的接触暴露毒性研究.结果显示,随着TBBPA的暴露浓度从0μmol/L增加至50μmol/L,两种细胞的存活率均逐渐下降,细胞通透性不断提高.此外,流式分析也表明了细胞在暴露后出现了部分凋亡,且随着浓度的提高,凋亡率也随之提高.进一步通过基因表达测定分析发现,与细胞凋亡相关基因p53,Survivin, bax, bcl-2, Caspase-3, Caspase-9的表达量均出现不同程度的上调,其中24 h的暴露相较于12 h会激发更为强烈的表达上调.此外,对暴露过程中细胞胞内活性氧(ROSs)组分、抗氧化性酶SOD和CAT活力活性进行了测定.两种细胞内的ROSs组分和抗氧化性酶均出现明显的提高,表明细胞在TBBPA暴露过程中收到了一定的氧化损伤并出现了氧化应激.Tetrabromobisphenol A(TBBPA) is a high-production chemical widely used in printed circuit boards and other polymeric materials for inflaming. As an environmental pollutant, it is widely found in various abiotic and biological media. Usually,the TBBPA could enter the body through skin contact, respiratory exposure and the dietary intake, among which respiratory exposure may be a most important exposure route. Previous studies reported that TBBPA possesses the endocrine, liver and kidney toxicity. However, few studies focused on the effects of TBBPA on respiratory toxicity. Therefore, it is urgent to study the toxic effects of TBBPA on respiratory system. In this study, human bronchial cells Beas2 B and pulmonary epithelial cells 16 HBE were used as subjects to study the exposure toxicity of TBBPA. Firstly, the viability and permeability of cells were assayed during the TBBPA exposure process. When TBBPA was in low concentration range(0-0.4 μmol/L), 24 h of exposure cannot significantly affect cell viability. When concentration of TBBPAwas greater than10 μmol/L, the inhibitory effect on cell activity was obviously observed. When concentration of TBBPA was up to50 μmol/L, the cell viabilities of 16 HBE and Beas2 B cells decreased to 80.9% and 86.2%. It is worth noting that, as compared with bronchial epithelial cell 16 HBE, lung epithelial cell Beas2 B has a higher tolerance to TBBPA, which may be due to the different cell structures.In addition, the oxidation stress of cells was also determined by assaying the cell intracellular reactive oxygen species(ROSs), antioxidant enzymatic activity of superoxide dismutase(SOD) and catalase(CAT) during the whole expose duration. The results showed that the levels of intracellular ROSs obviously increased in both cells, indicating that peroxidation occurred in the cells during TBBPA exposure. Correspondingly, the enzymatic activities of SOD and CAT also increased, indicating that oxidative stress happened in cells.After that, flow cytometry was applied to analyze cell apop

关 键 词：四溴双酚A 细胞毒性 细胞凋亡 氧化应激 基因表达上调 

分 类 号：R114[医药卫生—卫生毒理学]