某蛋鸡养殖场鸡滑液囊支原体的分离鉴定及跗关节病理组织学观察  

Isolation and Identification of Chicken Mycoplasma of in a Layer Farm and Histopathological Analysis of Hock Joints

作　　者：郭亚男[1] 郭朋辉 司朵朵 郭磊 何生虎[1] GUO Ya-nan;GUO Peng-hui;SI Duo-duo;GUO Lei;HE Sheng-hu(College of Agriculture,Ningxia University,Yinchuan,Ningxia,750021,China;Ningxia Xiaoming Agriculture and Animal Husbandry Co.Ltd.,Yinchuan,Ningxia,750000,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏晓鸣农牧股份有限公司,宁夏银川750000

出　　处：《动物医学进展》2020年第4期48-52,共5页Progress In Veterinary Medicine

基　　金：宁夏回族自治区重点研发计划项目(2019BBF02021);宁夏大学研究生创新项目(GIP2018042)。

摘　　要：用改良Frey培养基对采集的跗关节渗出物进行病原分离,设计滑液囊支原体16S rRNA通用引物和vlhA特异性引物进行基因序列扩增并测序,用MEGA6.0软件中的Neighbor-joining法依据16S rRNA和vlhA序列构建分离株系统发育树进行遗传进化分析;对采集的跗关节组织进行固定和脱钙处理后制作石蜡切片,HE染色镜检。结果发现,分离的病原菌落呈油煎蛋样;依据16S rRNA和vlhA基因序列构建的系统发育树与滑液囊支原体在同一分支;病变的跗关节组织主要出现滑膜上皮增厚,炎性细胞渗出,血管轻度增生,血细胞渗出和滑膜组织脱落等病变。表明引发鸡群发病的病原为滑液囊支原体,为临床和实验室诊断滑液囊支原体引起的疾病提供了参考。In order to isolate and identify the causative pathogen of Mycoplasma synoviae in a layer farm,and to analyze the damage of the pathogen to the hock joints,the improved Frey medium was used to isolate the pathological exudate.The 16S rRNA universal primers and vlhA specific primers were designed for the gene amplifications and sequencing.The NJ method of MEGA 6.0 software was used to construct the phylogenetic trees based on 16S rRNA and vlhA sequences and genetic evolution analysis.The collected joint tissues were fixed and decalcified,and then formed into paraffin sections and observed by HE staining.The results showed that the isolated pathogen colonies were fried egg-like;the phylogenetic trees constructed according to the 16S rRNA and vlhA gene sequences were in the same branch as Mycoplasma synoviae.Histopathological lesions,such as cell exudation,mild hyperplasia of blood vessels,blood cell exudation and synovial tissue shedding were observed.This study showed that the pathogen causing the disease of the flock was Mycoplasma synoviae,and provided a scientific basis for clinical and laboratory diagnosis of diseases caused by Mycoplasma synoviae.

关 键 词：鸡 滑液囊支原体 鉴定 跗关节 组织病理学 

分 类 号：S852.62[农业科学—基础兽医学]