基于荧光分子开关和核酸适配体的镉离子快速检测方法  

Rapid detection of cadmium based on fluorescence molecular switch and aptamer

作　　者：涂东堃 鲁悦 郑晓亮 黄敏丽 王奕婧 曾绍校[1,2,3] 徐晖 TU Dong-kun;LU Yue;ZHENG Xiao-liang;HUANG Min-li;WANG Yi-jing;ZENG Shao-xiao;XU Hui(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition,Ministry of Education,Fuzhou,Fujian 350002,China;Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]闽台特色海洋食品加工及营养健康教育部工程研究中心,福建福州350002 [3]福建省特种淀粉品质科学与加工技术重点实验室,福建福州350002

出　　处：《食品与机械》2020年第3期63-68,共6页Food and Machinery

基　　金：福建省教育厅省中青年教师教育科研项目(编号:JAT170195);福建省科技厅青年创新项目(编号:2019J05046)。

摘　　要：考查了不同pH值、噻唑橙—适配体浓度比、噻唑橙与适配体结合时间、适配体与镉离子结合时间对体系荧光强度的影响。结果表明:荧光检测的最佳条件为pH值7、噻唑橙—适配体浓度比7∶3、噻唑橙与适配体结合时间15min、适配体与镉离子结合时间20min。该条件下,荧光强度与镉离子浓度表现出良好的线性关系,线性范围为2.00~80.00ng/mL,检测限为0.23ng/mL。该方法不需要对适配体进行荧光标记,操作简单。In order to study the fluorescent dye thiazole orange as a fluorescent molecular switch and a cadmium ion nucleic acid aptamer for the detection of cadmium ions.The effects were examined,from different pH values,thiazole orange-aptamer concentration ratio,binding time of thiazole orange to aptamer,and binding time of aptamer to cadmium ion on the fluorescence intensity of the system.The results showed that when the pH value was 7,the thiazole orange-aptamer concentration ratio was 7∶3,the binding time of thiazole orange and aptamer was 15 min,and the binding time of aptamer and cadmium ion was 20 min.Under these conditions,the fluorescence intensity and cadmium ion concentration showed a good linear relationship,with a linear range from 2.00 ng/mL to 80.00 ng/mL,and the detection limit was 0.23 ng/mL.This method does not require fluorescent labeling of the aptamer,and the operation is simple.

关 键 词：噻唑橙 核酸适配体 荧光检测 镉离子 

分 类 号：X832[环境科学与工程—环境工程] O657.3[理学—分析化学]