雄蚕益肾方对肝郁肾虚ED大鼠阴茎eNOS、cGMP表达的影响  

Xiongcan Yishen Prescription upregulates the expressions of eNOS and cGMP in the penile tissue of ED rats with liver depression and kidney deficiency

作　　者：李波男 周海亮 肖丹[1] 何清湖[1] 周兴[3] LI Bo-nan;ZHOU Hai-liang;XIAO Dan;HE Qing-hu;ZHOU Xing(Hunan University of Chinese Medicine,Changsha,Human 410208,China;School of Integrated Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha,Human 410208,China;Department of Andrology,The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Human 410007,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学中西医结合学院,湖南长沙410208 [3]湖南中医药大学第一附属医院男科,湖南长沙410007

出　　处：《中华男科学杂志》2020年第2期167-173,共7页National Journal of Andrology

基　　金：国家自然科学基金(81202706,81673984,81774324);湖南省自然科学基金(2018JJ3402);湖南省教育厅优秀青年项目(16B200);长沙市杰出创新青年人才项目(kql802015)。

摘　　要：目的:探讨雄蚕益肾方对肝郁肾虚ED大鼠阴茎eNOS、cGMP表达的影响. 方法:50只8周龄SPF级雄性SD大鼠随机分为空白组,模型组,西药对照组,中成药对照组和雄蚕益肾方低、中、高剂量组,从50只雄性SD大鼠中随机抽取10只为空白组大鼠,其余大鼠均采用氢化可的松注射液肌注加束缚四肢复合造模方法连续14 d诱发肝郁肾虚ED模型.造模成功后,每组5只,西药对照组给予他达拉非片溶液0.52 mg,/(kg·d),中成药对照组给予疏肝益阳胶囊溶液0.3 125 g/(kg·d),雄蚕益肾方低、中、高剂量为10.4、20.8、41.6 g/(kg·d),空白组、模型组给予等体积蒸馏水,各组每日均早、晚各灌胃一次,连续28 d.造模前、造模完成后、给药28 d后均进行大鼠悬尾试验和交配实验.给药后第29天,以10%水合氯醛(3 ml/kg)麻醉大鼠,摘取大鼠阴茎组织,用免疫组化平均光密度值分析方法测定阴茎组织中eNOS、cGMP表达含量. 结果:模型组,雄蚕益肾方高、中、低剂量组和疏肝益阳组悬尾不动时间分别为(3.17±0.11)、(2.58 ±0.25)、(2.52 ±0.31)、(2.51±0.3)、(2.57±0.29) min,与模型组相比较,雄蚕益肾方高、中、低剂量组和疏肝益阳组显著降低(P<0.05).模型组,雄蚕益肾方高、中、低剂量组,疏肝益阳组,他达拉非组骑跨潜伏期(ML)分别为(9.23±0.11)、(1.21±0.12)、(2.17±0.16)、(2.26±0.13)、(1.23±0.15)、(2.48 ±0.18) min,骑跨次数(MF)分别为(0.48±0.18)、(3.29±0.11)、(3.18±0.11)、(3.05±0.05)、(3.23±0.12)、(3.2±0.28)次,插入次数(IF)分别(0.8±0.84)、(11.8±0.84)、(11.2±1.48)、(9.4±1.14)、(11.4±1.14)、(10±1.22)次,与模型组相比较,雄蚕益肾方高、中、低剂量组,疏肝益阳组,他达拉非组ML显著减少,MF、IF显著增加(P<0.05).eNOS、cGMP主要表达于阴茎海绵体动、静脉内皮细胞及海绵体窦状隙内皮细胞的细胞核和细胞质内,呈棕黄色颗粒,散在、局灶性表达;与模型组相比较,雄蚕益肾方高、�Objective:To investigate the effect of Xiongcan Yishen Prescription(XYP)on the expressions of eNOS and cGMP in the penile tissue of ED rats with liver depression and kidney deficiency(LDKD).Methods:The model of ED-LDKD was established in 30 eight-week-old SPF-class male SD rats by injecting hydrocortisone intramuscularly and binding the limbs for 14 days,and another 10 rats were taken as blank controls.Then,the model rats were randomized into six groups of equal number and treated intragastrioally with distilled water(model control),tadalafil tablets at 0.52 mg/kg/d(tadalafil control),Shugan Yiyang Capsules 0.3125 g/k g/d(SYC control),and XYP at 10.4 g/k g/d(low-dose XYP),20.8 g/k g/d(medium-dose XYP)and 41.6 g/k g/d(high-close XYP),bid,for 28 successive days,respectively.Before and after modeling and after 28-day treatment,the animals were subjected to tail suspension and imiting tests.The next day after medication,ihe penile tissues of the rats were harvested for determining the expression levels of eNOS and cGMP proteins by imnuinohistochemical analysis of the mean optical density.Results:Compared with the model controls,the rats of the high-,medium-and low-dose XYP and SYC control groups all showed significant decreases in the tail suspension time([3.17±0.11]vs[2.58±0.25],[2.52±0.31],[2.51±0.3]vs[2.57±0.29]min,P<0.05)and mount latency(ML)([9.23±0.11]ys[1.21±0.12],[2.17±0.16],[2.26±0.13],[1.23±0.15]and[2.48士0.18]min,P<0.05)but increases in mount frequency(MF)([0.48±0.18]vs[3.29±0.11],[3.18±0.11],[3.05±0.05],[3.23±0.12]and[3.2±0.28]times,P<0.05)and intromission frequency(IF)([0.8±0.84]vs[11.8±0.84],[11.2±1.48],[9.4±1.14],[11.4±1.14]and[10±1.22]tim es,P<0.05).The eNOS ami cGMP proteins were mainly expressed in the nucleus and cytoplasm oi the arterial and venous endothelial cells and sinusoidal endothelial cells of the cavernous,as brownish yellow particles in a scattered and focal pattern.Both the expressions of eNOS and cGMP in the penile tissue were remarkably upregulated in the high-,

关 键 词：雄蚕益肾方 肝郁肾虚 勃起功能障碍 内皮型一氧化氮合酶 环磷酸鸟苷 

分 类 号：R698.1[医药卫生—泌尿科学]