细辛叶枯病生防真菌的筛选、鉴定及拮抗效果评价  

Screening, identification and antagonistic effect evaluation of biocontrol fungi to Asarum leaf blight

作　　者：刘亚苓 于营[1] 崔丽丽[1] 雷慧霞 鲁海坤 张新静[1] 郭靖[1] Liu Yaling;Yu Ying;Cui Lili;Lei Huixia;Lu Haikun;Zhang Xinjng;Guo Jing(Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun Jilin 130112,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112

出　　处：《中国植保导刊》2020年第3期12-18,共7页China Plant Protection

基　　金：国家重点研发计划子课题(2016YFC0500303-07);吉林省科技发展计划项目(20180201034YY);中国农业科学院科技创新工程(CAAS-ASTIP-2016-ISAPS)。

摘　　要：通过平板对峙培养和发酵液抑菌试验,从细辛健康植株根际土壤中筛选出拮抗效果较好的生防真菌F1-6菌株,经形态学和ITS序列分析鉴定,确定该菌为微紫青霉(Penicillium janthinellum)。对峙培养和发酵液抑菌试验结果表明,该菌株对叶枯病病菌的抑菌率分别为61.25%和63.64%;室内盆栽防治试验结果表明,该菌株对细辛叶枯病的预防效果为70.78%、治疗效果为61.03%。The biocontrol fungi with antagonistic effect to Asarum leaf blight were separated from the rhizosphere soil of healthy Asarum and screened by the plate confrontation and fermentation broth inhibition test. A biocontrol fungus F1-6 with a high antagonistic effect was selected. After morphological and ITS sequence analysis, it was identified as Penicillium janthinellum. The fungal inhibition rates in plate confrontation and fermentation broth inhibition tests were61.25% and 63.64%, respectively. The results of the pot experiment showed that the prevention effect of this strain on Asarum leaf blight was 70.78%, and its treatment effect was 61.03%, showing occurrence-preventing and severity-reducing efficiency against the disease.

关 键 词：细辛叶枯病 生防真菌 微紫青霉 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]