人胚胎发育过程中间充质干祖细胞与造血细胞间的起源关系探讨  

Developmental relationship between mesenchymal stem/progenitor cells and hematopoietic cells during human embryogenesis

作　　者：王长振 王凤华[3] 高娇 江小霞 李红[1] 朱恒 毛宁 王晓燕 Wang Changzhen;Wang Fenghua;Gao Jiao;Jiang Xiaoxia;Li Hong;Zhu Heng;Mao Ning;Wang Xiaoyan(Institute of Military Cognition and Brain Science,Academy of Military Medical Sciences,Beijing 100850,China;Beijing Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China;Department of Clinical Laboratory,Qingdao Municipal Hospital,Qingdao 266011,China)

机构地区：[1]军事科学院军事医学研究院军事认知与脑科学研究所,北京100850 [2]军事科学院军事医学研究院辐射医学研究所,北京100850 [3]青岛市市立医院检验科,266011

出　　处：《中华微生物学和免疫学杂志》2020年第1期19-24,共6页Chinese Journal of Microbiology and Immunology

基　　金：北京市自然科学基金(7172151);国家自然科学基金(81170461,81070393)。

摘　　要：目的探讨人胚胎发育过程中间充质干祖细胞(MSPCs)与造血细胞间的起源关系。方法取发育不同时间药流胚胎,分离不同造血组织消化成单个细胞,于高增殖潜能集落形成细胞(HPP-CFC)培养体系培养10~14 d,倒置显微镜下挑取直径大于0.5 mm的HPP-CFC集落于液体培养体系进行二次培养,对二次培养中出现的贴壁细胞进行扩增并鉴定其细胞表面分子的表达,于不同分化体系鉴定其是否具有MSPCs的分化特性。结果本研究总结了胚胎发育不同时期主动脉-性腺-中肾(AGM)区、卵黄囊、胎肝等不同部位包括HPP-CFC在内的各类造血前体细胞的发育动态,发现从28体节开始,一定比例的AGM区HPP-CFC除能够分化产生造血细胞外,其来源的贴壁细胞具有MSPCs的分化功能,贴壁细胞在淋巴母细胞转化实验中可抑制T细胞的增殖。结论人胚胎AGM区内,部分MSPCs和造血细胞起源于共同前体。Objective To analyze the developmental relationship between mesenchymal stem/progenitor cells(MSPCs)and hematopoietic cells during human embryogenesis.Methods Aborted embryos at different developmental stages were used in this study after medical abortion.Embryonic blood tissues were isolated and digested into single cells.These single cells were plated in semisolid medium in favor of the differentiation of colony-forming cell with high proliferative potential(HPP-CFC)and incubated for 10 to 14 d.Individual colonies with diameter more than 0.5 mm were picked and replated in liquid medium.Fibroblastic adherent cells appeared in the replated colonies were cultured for cell proliferation and cytokins expressed on cell surface were identified to analyze whether they had the characteristics of MSPCs.Results This study summarized the dynamic development of HPP-CFCs and other hematopoietic progenitor cells in different tissues including aorta-gonad-mesonephros(AGM)region,yolk sac and embryonic liver.From the 28-somite stage,a proportion of HPP-CFCs in AGM region could give rise to adherent fibroblastic cells in addition to hematopoietic cells.The adherent cells harbored the differentiation potential of MSPCs and could inhibit the proliferation of T cells in lymphocyte transformation test.Conclusions This study suggests some prehematopoietic precursors in AGM region can give rise to both hematopoietic progenitors and MSPCs during human embryogenesis.

关 键 词：间充质干祖细胞 高增殖潜能集落形成细胞 造血细胞 免疫抑制 淋巴母细胞转化试验 

分 类 号：R3[医药卫生—基础医学]