青藏高原不同生态环境下冰川棘豆(Oxytropis glacialis)内生细菌多样性研究  

Study on endophyte diversity of Oxytropis glacialis in different ecological environments in Qinghai-Tibet Plateau

作　　者：许国琪 刘怡萱[1,2] 曹鹏熙 刘星 XU Guoqi;LIU Yixuan;CAO;Pengxi;LIU Xing(School of Science,Tibet University,Lhasa 850000,China;Laboratory of Adaptation and Evolution of Plateau Biota in Extreme Environment,Research Center for Ecology and Environment of Qinghai-Tibetan Plateau,Tibet University,Lhasa 850000,China;College of Life Sciences,Wuhan University,Wuhan 430072,China)

机构地区：[1]西藏大学理学院,西藏拉萨850000 [2]西藏大学青藏高原生态与环境研究中心极端环境生物适应与进化实验室,西藏拉萨850000 [3]武汉大学生命科学学院,湖北武汉430072

出　　处：《高原科学研究》2020年第1期20-29,共10页Plateau Science Research

基　　金：2018年中央财政支持地方高校改革发展专项资金(藏财教指〔2018〕54号);2019年中央财政支持地方高校改革发展专项资金(藏财教指〔2019〕01号);西藏大学研究生高水平人才培养计划项目(2017-GSP-114).

摘　　要：植物内生菌(Endophyte)有助于宿主植物生存与扩散。文章利用高通量测序技术,对青藏高原高寒草原区(TAS)和灌丛草甸区(TAM)冰川棘豆内生细菌多样性进行分析,为疯草类有毒植物的适应性研究提供基础参考。测序得到236205条有效序列,注释到476个OTUs,隶属17个门281个属。多样性分析表明,区域间菌群结构无显著性差异。组成分析显示,不同样本间优势菌门均为变形菌门(Proteobacteria),假单胞菌属(Pseudomonas)、Lachnoclostridium 5和泛菌属(Pantoea)等18个菌属为优势菌属。环境分析认为,土壤电导率(EC)、空气CO2浓度(CC)和土壤温度(ST)对菌群结构的差异具有不同程度的显著性影响,56%的优势菌属与环境因子显著相关。功能预测分析认为,KEGG代谢通路和COG各功能相关的基因丰度在不同区域样本间相似,参与多种宿主代谢调控。综上所述,不同区域间冰川棘豆内生细菌菌群结构及功能的宿主保守性大于地域特异性,在宿主植物中参与各种代谢网络调控。相对稳定的菌群结构及优势菌属丰度随环境显著变化,这有利于冰川棘豆在不同的生态环境中生存和扩散。Endophyte helps survive and spread of host plants. In this study, the diversity of endophytetic bacteria of Oxytropis glacialis distributed at alpine steppe(TAS) and alpine shrub meadow(TAM) was analyzed in Tibet with high-throughput screening technology, so as to provide a basic reference for the study on the adaptability of the poisonous plants. Total 236 205 sequences and 476 OTUs were obtained belonging to 17 phyla and 281 genera. There were no significant differences between the TAM and the TAS samples. Composition analysis showed that the dominant phyla in different samples were Proteobacteria, Pseudomonas, Lachnoclostridium 5, Pantoea and other 13 genera were the predominant genera. According to analysis of the environmental factors, soil conductivity(EC), air CO2 concentration(CC) and soil temperature(ST) have different degrees of significant effect on the different bacterial community structure, and 56% of dominant bacteria were significantly related to environmental factors. Functional prediction analysis suggested that the abundance of genes related to KEGG metabolic pathway and COG was similar among samples from different regions, and involved in multiple host metabolic regulation. It can be concluded that the host plant conservative performance of relative stable bacterial community structure and function in Oxytropis glacialis between regions was larger than regional specificity and involved in the regulation of various metabolic networks in host plants. The relative stable bacterial community structure and abundance of the dominant genus changed with the environmental factors significantly, which is conducive to the survival and spread of host plant in different ecological environments.

关 键 词：冰川棘豆 植物内生细菌 16S rRNA 土壤理化因子 PICRUSt KEGG COG 

分 类 号：Q945[生物学—植物学]