在线液相色谱-气相色谱/质谱联用快速分析烟叶中的西柏三烯二醇  

Analysis of cembratriene-diols in tobacco by LC-GC/MS

作　　者：黄志 毕艳玖 沙云菲 谢雯燕 吴达 刘百战 刘太昂[2] HUANG Zhi;BIYanjiu;SHA Yunfei;XIE Wenyan;WU Da;LIU Baizhan;LIU Taiang(Technical Center,Shanghai Tobacco Group Co.,Ltd.,Shanghai 200082,China;School of Materials Science and Engineering,Shanghai University,Shanghai 200444,China)

机构地区：[1]上海烟草集团技术中心,上海200082 [2]上海大学材料科学与工程学院,上海200444

出　　处：《上海大学学报（自然科学版）》2020年第2期227-233,共7页Journal of Shanghai University:Natural Science Edition

基　　金：国家重点研究发展计划专项资助项目(2016YFB0700504);中国烟草总公司科技重大专项资助项目(中烟办[2016]259号)。

摘　　要：西柏三烯二醇是重要的中性香味物质--茄酮及其衍生物的前体物,对烟叶的香气风格和品质具有重要影响.建立了以超声波辅助溶剂萃取技术,结合在线液相色谱-气相色谱/质谱(liquid chromatography-gas chromatography/mass spectrometry,LC-GC/MS)联用法对烟草中的西柏三烯二醇进行快速分析的方法.该方法的前处理操作快速简便,检测灵敏度高,重复性好,在对不同地区K326青烟叶中西柏三烯二醇含量的比较分析中也取得了良好的结果,适用于烟草中西柏三烯二醇含量的大规模分析.Cembratriene-diols are the precursors of important neutral aroma components and their derivatives,which affect significantly the aroma style and tobacco quality.In this study,a method of in-series combination of ultrasound-assisted extraction and online liquid chromatography-gas chromatography/mass spectrometry(LC-GC/MS)was constructed for the analysis of cembratriene-diols in tobacco.Cembratriene-diols were first extracted by ultrasound-assisted extraction and then on-line clean-up and analysis by online LCGC/MS system.The pretreatment was highly efficient and easy-handling.Good recovery and reproducibility had also been achieved.This method was subsequently applied to the analysis of cembratriene-diols extracted from different regions of tobacco leaves.The satisfactory performance indicated that this strategy was suitable for the high-throughput analysis of cembratriene-diols in tobacco.

关 键 词：烟叶 西柏三烯二醇 硅烷化衍生 液相色谱-气相色谱/质谱 

分 类 号：O657.63[理学—分析化学]