基于元分析的大豆胞囊线虫病3号生理小种抗性基因挖掘  

Mining of resistance genes of soybean cyst nematode Race 3(HG Type 0, SCN Race 3) by meta-analysis

作　　者：韩英鹏[1] 田利峥 姜海鹏 包冬芳 王俊 赵雪[1] HAN Yingpeng;TIAN Lizheng;JIANG Haipeng;BAO Dongfang;WANG Jun;ZHAO Xue(Institute of Soybean Research,Key Laboratory of Soybean Biology in Chinese of Ministry Education,Key Laboratory of Soybean Biology and Breeding(Genetics)of Ministry of Agriculture and Rural Affairs,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学大豆研究所,大豆生物学教育部重点实验室,农业农村部东北大豆生物学与遗传育种重点实验室,哈尔滨150030

出　　处：《东北农业大学学报》2020年第4期10-17,共8页Journal of Northeast Agricultural University

基　　金：黑龙江省杰出青年基金项目(JC2018007);国家自然科学基金面上项目(31671717,31971967);国家科技重大专项和重点研发项目(课题)省级资助项目(GX17B002);黑龙江省博士后项目(LBH-Q17015);国家重点研发计划项目(2016YFD0100304)。

摘　　要：大豆胞囊线虫病(Soybean cyst nematode,Heterodera glycines Ichinohe)是一种严重影响大豆产量的病害,我国东北地区以3号生理小种(SCN Race 3)为主。QTL定位是挖掘抗性基因最有效的方法之一,与SCN相关的QTL报道较多,其由不同遗传群体获得,但在不同遗传群体间较难重复。研究采用图谱整合软件BioMercatorV4.2挖掘SCN Race 3抗性基因。将收集整理的10篇文献发表的33个QTL整合在遗传图谱GmComposite2003上,通过元分析方法得到一致性QTL,最终获得3个抗性候选区段,发现18个SCN抗性基因,其中17个PK型基因和1个LRR型基因,分别位于7、15和18染色体中。对潜在SCN抗性基因分析蛋白结构域发现,17个PK型基因分别含有丝/苏氨酸蛋白激酶活性、钙依赖蛋白激酶活性、分裂原活化蛋白激酶活性、半胱氨酸蛋白激酶和组/精/赖氨酸蛋白激酶活性。这些基因编码蛋白结构域与植物抗病相关,结果可为抗SCN基因挖掘和选育抗SCN品种(系)提供理论基础。Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is a serious disease affecting soybean yield.SCN Race 3 is dominant in northeast China.QTL mapping is one of the most effective methods for mining resistance genes;however,these QTLs were obtained from different genetic populations and are difficult to repeat among different genetic populations.In this study,map integration software BioMercatorV4.2 was used to mine SCN Race 3 resistance genes.The total 33 QTL published in 10 articles were integrated into the genetic map GmComposite2003.Additionally,the consistent QTLs were obtained by meta-analysis,and finally three resistance candidate segments were obtained,and 18 SCN resistance genes,17 PK type genes and 1 LRR type gene were found,which were located in 7,15 and 18 chromosomes.Protein domain analysis of potential SCN resistance genes showed that 17 PK genes contained serine/threonine protein kinase activity,calcium dependent protein kinase activity,mitogen activated protein kinase activity,cysteine protein kinase activity and group/arginine protein kinase activity.Combined with the previous research results,it was shown that the domain of proteins encoded by these genes was related to plant disease resistance,which provided a theoretical basis for SCN-resistant gene mining and breeding of SCN-resistant varieties(lines).

关 键 词：大豆胞囊线虫病 QTL 抗性位点 元分析 候选基因 

分 类 号：S565.1[农业科学—作物学]