鸡HMG box蛋白1(HBP1)基因启动子区多态性与腹脂率关联分析  

Association between polymorphism of HMG box protein 1(HBP1)gene with abdominal fat percentage in chickens

作　　者：王守志 张长超[1,2,3] 李紫薇 王伟佳 李玉东 王宁 李辉 WANG Shouzhi;ZHANG Changchao;LI Ziwei;WANG Weijia;LI Yudong;WANG Ning;LI Hui(Key Laboratory of Chicken Genetics and Breeding,Ministry of Agriculture and Rural Affairs,Harbin 150030,China;Key Laboratory of Animal Genetics,Breeding and Reproduction,Education Department of Heilongjiang Province,Harbin 150030,China;School of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]农业农村部鸡遗传育种重点实验室,哈尔滨150030 [2]黑龙江省普通高等学校动物遗传育种与繁殖重点实验室,哈尔滨150030 [3]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《东北农业大学学报》2020年第4期55-60,共6页Journal of Northeast Agricultural University

基　　金：国家自然科学基金面上项目(31572394);现代农业产业技术体系建设专项(CARS-41)。

摘　　要：为探讨HMG box蛋白1(HMG box protein 1,HBP1)基因启动子区多态性对鸡腹脂率的影响,以东北农业大学高、低脂双向选择品系第十九世代肉鸡为试验材料,采用直接测序法检测HBP1基因多态性和个体基因型,共获得168个SNPs位点基因型。应用滑动窗口法对位于该基因启动子区54个多态性位点构建单倍型,分别开展单位点和单倍型与鸡腹脂率的关联分析。结果表明,HBP1基因启动子区有7个(g.-5335G>A、g.-4354T>A、g.-3328C>T、g.-3327G>A、g.-1013T>C、g.-990G>A和g.-159A>C)位点显著影响肉鸡腹脂率(P<0.05)。此外,等位基因频率分析显示,这些位点等位基因频率在两系间分布存在极显著差异(P<0.0001)。研究结果表明,HBP1基因为影响鸡腹脂率重要候选基因,7个多态性位点为影响鸡腹脂率重要分子标记。In order to investigate the effects of polymorphisms of HMG box protein 1(HBP1)gene on abdominal fat percentage in chickens,the19 th generation population of the chicken divergently selected for abdominal fat content of Northeast Agricultural University(NEAUHLF)was used in the present study.The direct sequencing was used for the detection of HBP1 gene polymorphisms and individual genotyping.A total of 168 SNPs were found.Fifty-four SNPs on the promoter region were applied to construct haplotypes using sliding window method,association of individual SNP genotypes and haplotypes with chicken abdominal fat percentage was analyzed,respectively.The results showed that there were seven loci(g.-5335 G>A,g.-4354 T>A,g.-3328 C>T,g.-3327 G>A,g.-1013 T>C,g.-990 G>A and g.-159 A>C)on the promoter of HBP1 gene that significantly affected abdominal fat percentage(P<0.05).In addition,analysis of SNP alleles showed that significant differences in allele frequencies of seven SNPs between two lines were observed(P<0.0001).The findings suggested that HBP1 gene could be an important candidate gene responsible for abdominal fat in chickens,and seven SNPs were important molecular markers for abdominal fat percentage in chickens.

关 键 词：肉鸡 腹脂率 HBP1基因 SNPS 

分 类 号：S831[农业科学—畜牧学]