甜瓜柱头黄绿颜色相关性状QTL分析  

QTL Analysis of Melon Stigma Color

作　　者：郭禹 郭海艳 罗澜 刘识 高业 张泰峰 栾非时 王学征 GUO Yu;GUO Hai-yan;LUO Lan;LIU Shi;GAO Ye;ZHANG Tai-feng;LUAN Fei-shi;WANG Xue-zheng(College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]东北农业大学园艺园林学院,黑龙江哈尔滨150030

出　　处：《中国蔬菜》2020年第5期26-33,共8页China Vegetables

基　　金：国家西甜瓜产业技术体系项目(CARS-026-02);国家自然科学基金项目(31772331)。

摘　　要：以绿色柱头甜瓜品种MR-1和黄色柱头甜瓜品种TN为亲本,构建F2群体,利用200个F2单株为作图群体,基于双亲材料基因组重测序数据开发的SNP-CAPS标记,构建了一张包含12个连锁群、135个CAPS标记的遗传连锁图谱,覆盖基因组长度2731.97 cM。利用该图谱对甜瓜柱头黄绿颜色相关性状进行QTL分析,共检测到12个与柱头颜色相关的QTL,其中与柱头总叶绿素含量相关QTL 4个,柱头类胡萝卜素含量相关QTL 3个,柱头色卡颜色相关QTL 2个,柱头总叶绿素含量与类胡萝卜素含量比值相关QTL 3个,分布在1、2、4、5、7、8、11、12号连锁群上,可解释4.63%~20.44%的表型变异,其中,调控柱头总叶绿素含量、类胡萝卜素含量和柱头色卡颜色的主效位点分别为SChC-1-1、SCaC-12-1和SCoC-2-1,其贡献率分别为18.78%、12.08%和20.44%。In this experiment,200 F2 populations were obtained from green stigma melon‘MR-1’and yellow stigma melon‘TN’as parents,and based on resequencing the SNP-CAPS markers and BSA technology were developed for construction of the molecular map.A genetic linkage map containing 12 linkage groups and 135 CAPS markers was constructed,covering a genome length of 2731.97 cM.QTL analysis was conducted on the related characters of melon stigma color(yellow or green)by this map.Twelve QTLs related to stigma color were obtained.There were 4 QTLs related to stigma total chlorophyll content,3 QTLs related to stigma carotenoid content,2 QTLs related to stigma color card,and 3 QTLs related to the ratio of stigma total chlorophyll content to stigma carotenoid content,distributing in 1,2,4,5,7,8,11,12 on the linkage group,explaining 4.63%-20.44%phenotypic variation.The main effect sites regulating stigma total chlorophyll content,stigma carotenoid content and stigma color card were SChC-1-1,SCaC-12-1and SCoC-2-1.Their contribution rates were 18.78%,12.08%,20.44%,respectively.

关 键 词：甜瓜 遗传图谱 柱头颜色 QTL 定位 

分 类 号：S652[农业科学—果树学]