替考拉宁高效液相色谱手性固定相的制备研究  

Preparation of chiral teicoplanin stationary phase for HPLC

作　　者：李丹 李倩 段爱红[1] 袁黎明[1] LI Dan;LI Qian;DUAN Aihong;YUAN Liming(College of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,Yunnan,China)

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《化学研究》2019年第6期593-598,共6页Chemical Research

基　　金：国家自然科学基金(21665028).

摘　　要：替考拉宁属于大环抗生素,具有半篮状结构和多个手性中心,是常见的手性识别材料,广泛应用于对映体的色谱手性分离分析.本文研究了以替考拉宁为手性识别剂,采用键合的方法制备得到9种高效液相色谱手性固定相,用于苯甘氨酸和对羟基苯甘氨酸的拆分研究,并且考察了重现性和稳定性及进样量对拆分结果的影响.实验结果表明,9种手性固定相均具有拆分苯甘氨酸及对羟基苯甘氨酸的能力.Teicoplanin is a macrocyclic antibiotic with half basket shape structure and multiple chiral centers.It is a common chiral recognition material and widely used in chromatographic chiral separation and analysis of enantiomers.In this work,9 kinds of chiral stationary phases were prepared with the teicoplanin as chiral recognition agent by bonding method,which were subsequently used for the separation of phenylglycine and p-hydroxyphenylglycine.The results showed that all the 9 chiral stationary phases possess capable of separating phenylglycine and p-hydroxyphenylglycine.

关 键 词：替考拉宁 手性固定相 高效液相色谱 手性分离 

分 类 号：O657.7[理学—分析化学]