EGFR signaling augments TLR4 cell surface expression and function in macrophages via regulation of Rab5a activation  

作　　者：Jing Tang Bowei Zhou Melanie JScott Linsong Chen Dengming Lai Erica KFan Yuehua Li Qiang Wu Timothy RBilliar Mark AWilson Ping Wang Jie Fan 

机构地区：[1]The Department of Anesthesiology,Affiliated hospital of Guangdong Medical University,Zhanjiang 524000,China [2]Department of Surgery,University of Pittsburgh School of Medicine,Pittsburgh,PA 15213,USA [3]Research and Development,Veterans Affairs Pittsburgh Healthcare System,Pittsburgh,PA 15240,USA [4]Department of Anesthesiology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China [5]Department of Thoracic Surgery,Shanghai Pulmonary Hospital,Tongji University School of Medicine,Shanghai 200433,China [6]Department of Cardiovascular Surgery,The Children's Hospital of Zhejiang University School of Medicine,Hangzhou 310052,China [7]University of Pittsburgh The Graduate School of Public Health,Pittsburgh,PA 15213,USA [8]Laboratory of Tropical Biomedicine and Biotechnology,School of Tropical Medicine and Laboratory Medicine,Hainan Medical University,Haikou 571199,China [9]The Feinstein Institute for Medical Research,Manhasset,NY 11030,USA [10]McGowan Institute for Regenerative Medicine,University of Pittsburgh,Pittsburgh,PA 15219,USA [11]Key Laboratory of Organ Injury/Protection and Translational Medicine of Zhanjiang,Zhanjiang 524000,China

出　　处：《Protein & Cell》2020年第2期144-149,共6页蛋白质与细胞（英文版）

基　　金：This work was supported by the National Institutes of Health Grant R01-HL-079669(J.F.and M.A.W.);National Institutes of Health Grant R01-HL-139547(J.F.and M.A.W.);National Institutes of Health Grant R01HL076179(P.W.and J.F.);VA Merit Award 1I01BX002729(J.F.);VA BLR&D Research Career Scientist Award BX004211(J.F.);National Natural Science Foundation of China 81671957(J.T.);Key projects of Guangdong Natural Science Foundation 2018B030311038(J.T.);Science and Technology Planning Project of Guangdong Province 2016A020215212(J.T.);National Institutes of Health Grant R01GM102146(M.J.S).

摘　　要：Dear Editor,oll-like receptor 4(TLR4)is a key receptor sensing bacterial lipopolysaccharide(LPS),and is the most investigated member of the Tol-like receptor family(Kawai and Akira,2007;Kayagaki et al,2013;Klein et al.,2015).Cell surface TLR4 expression is determined by the balance between receptor trafficking from the Golgi apparatus to the cell membrane,and internalization of the cell surface receptor into endosomal compartments(Saltoh,2009).In bone mar-row-derived macrophages(BMDM),we observed LPS-in-duced EGFR phosphorylation on the surface of BMDM,and this was inhibited by pretreatment with PD168393 or TAPI-1(Fig.S1).Next,we measured dynamic changes in cell sur-face TLR4 expression after LPS treatment.At6,12,and 24 h after LPS treatment,TLR4 expression on the surface of BMDM was increased^2-,~6-,and^9-fold,respectively,as compared with controls.

关 键 词：PD1 TLR4 inhibited 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]