HPLC法同时测定感冒止咳颗粒中10种成分  

Simultaneous determination of ten constituents in Ganmao Zhike Granules by HPLC

作　　者：何艳 胡小祥 王庆 HE Yan;HU Xiao xiang;WANG Qing(College of Pharmacy,Xiangnan University,Chenzhou 423000,China;Chenzhou Municipal Center for Food and Drug Control,Chenzhou 423000,China)

机构地区：[1]湘南学院药学院,湖南郴州423000 [2]郴州市食品药品检验检测中心,湖南郴州423000

出　　处：《中成药》2020年第4期849-852,共4页Chinese Traditional Patent Medicine

基　　金：湖南省“双一流”药学应用特色学科基金(湘教通[2018]469)。

摘　　要：目的建立HPLC法同时测定感冒止咳颗粒(柴胡、山银花、葛根等)中新绿原酸、绿原酸、隐绿原酸、葛根素、连翘酯苷A、异绿原酸A、异绿原酸C、黄芩苷、汉黄芩苷、黄芩素的含有量。方法该药物70%甲醇提取液的分析采用Agilent ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 m L/min;柱温30℃;检测波长250、278、326 nm。结果 10种成分在各自范围内线性关系良好(r≥0.999 4),平均加样回收率为98.05%~101.95%,RSD为0.45%~2.28%。结论该方法简便准确,重复性好,可用于感冒止咳颗粒的质量控制。AIM To establish an HPLC method for the simultaneous content determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,puerarin,forsythiaside A,isochlorogenic acid A,isochlorogenic acid C,baicalin,wogonoside and baicalein in Ganmao Zhike Granules(Bupleuri Radix,Lonicerae Flos,Puerariae lobatae Radix,etc.).METHODS The analysis of 70%methanol extract of this drug was performed on a 30℃thermostatic Agilent ZORBAX SB-C18column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 m L/min in a gradient elution manner,and the detection wavelengths were set at 250,278,326 nm.RESULTS Ten constituents showed good linear relationships within their own ranges(r≥0.999 4),whose average recoveries were 98.05%-101.95%with the RSDs of 0.45%-2.28%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Ganmao Zhike Granules.

关 键 词：感冒止咳颗粒 化学成分 HPLC 

分 类 号：R927[医药卫生—药学]