UPLC法测定生、制白术配伍枳术丸中10种成分  

Determination of ten constituents in raw and processed Atractylodes macrocephala compatible Zhizhu Pills by UPLC

作　　者：臧彬如 单国顺 贾天柱[2] 张诗雯[2] 周改莲 ZANG Bin ru;SHAN Guo shun;JIA Tian zhu;ZHANG Shi wen;ZHOU Gai lian(Guangxi University of Traditional Chinese Medicine,Nanning 530001,China;Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]广西中医药大学药学院,广西南宁530001 [2]辽宁中医药大学,辽宁大连116600

出　　处：《中成药》2020年第4期960-964,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金青年基金项目(81803726);辽宁省自然科学基金博士启动项目(20180540012);辽宁省自然科学基金指导计划项目(20170540596)。

摘　　要：目的建立UPLC法测定生、制白术配伍枳术丸中橙皮苷、柚皮苷、芸香柚皮苷、柚皮素、橙皮素、辛弗林、荷叶碱、白术内酯Ⅱ、白术内酯Ⅰ、白术内酯Ⅲ中的含有量。方法枳术丸甲醇提取物的分析采用ACQUITY UPLC HSS T3色谱柱(2. 1 mm×100 mm,1. 8μm);流动相0. 1%甲酸-0. 1%甲酸乙腈,梯度洗脱;体积流量0. 4 m L/min;柱温30℃;283 nm波长下测定橙皮苷、柚皮苷、芸香柚皮苷、柚皮素、橙皮素、辛弗林、荷叶碱、白术内酯Ⅱ;220 nm波长下测定白术内酯Ⅰ、白术内酯Ⅲ。结果 10种成分在各自范围内线性关系良好(r>0. 999 0),平均加样回收率97. 5%~100. 6%,RSD 1. 89%~3. 16%。结论该方法准确稳定,重复性好,可用于枳术丸的质量控制。AIM To establish a UPLC method for the content determination of hesperidin, naringin,narirutin,naringenin,hesperetin,synephrine,nuciferine,atractylenolide Ⅱ,atractylenolide Ⅰ and atractylenolide Ⅲin raw and processed Atractylodes macrocephala Koidz. compatible Zhizhu Pills. METHODS The analysis of methanol extract of Zhizhu Pills was performed on a 30 ℃ thermostatic ACQUITY UPLC HSS T3 column(2. 1 mm×100 mm,1. 8 μm),with the mobile phase comprising of 0. 1% formic acid-0. 1% formic acid flowing at 0. 4 m L/min in a gradient elution manner,and the detection wavelengths of hesperidin,naringin,narirutin,naringenin,hesperetin,synephrine,nuciferine,atractylenolide Ⅱ and the detection wavelengths of atractylenolideⅠ,atractylenolide Ⅲ were set at 283,220 nm. RESULTS Ten constituents showed good linear relationships within their own ranges( r > 0. 999 0),whose average recoveries were 97. 5%-100. 6%, with the RSDs of1. 89%-3. 16%. CONCLUSION This accurate,stable and reproducible method can be used for the quality control of Zhizhu Pills.

关 键 词：枳术丸 生白术 制白术 化学成分 UPLC 

分 类 号：R284[医药卫生—中药学]