新型冠状病毒核酸检测临床实验室操作规范的建议  

Proposal for standardization of 2019-nCoV nucleic acid detection in clinical laboratories

作　　者：童永清[1] 汪明[1] 徐万洲[1] 乔斌[1] 郑红云[1] 梅四清[1] 何晓云[1] 张平安[1] 李艳[1] Tong Yongqing;Wang Ming;Xu Wanzhou;Qiao Bin;Zheng Hongyun;Mei Siqing;He Xiaoyun;Zhang Ping'an;Li Yan(Department of Clinical Laboratory,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院检验医学中心,武汉430060

出　　处：《中华检验医学杂志》2020年第3期209-212,共4页Chinese Journal of Laboratory Medicine

摘　　要：2019年12月暴发于湖北省武汉市的新型冠状病毒肺炎,2020年1月20日被纳入《中华人民共和国传染病防治法》规定的乙类传染病,并采取甲类传染病的预防、控制措施。2020年1月18日《新型冠状病毒感染的肺炎诊疗方案(试行第二版)》将新型冠状病毒核酸检测列为确诊标准,规范临床实验室新型冠状病毒核酸检测的操作流程成为当务之急,有必要制订针对新型冠状病毒核酸检测临床实验室操作规范,提高检测结果的可靠性,保证检测人员的安全。In December,the outbreak of a novel coronavirus(2019-nCoV)in Wuhan,China,has attracted extensive global attention.On January 20,2020,the Chinese health authorities upgraded the coronavirus to a Class B infectious disease in the Law of the People′s Republic of China on the Prevention and Treatment of Infectious Diseases,and considered it as Class A infectious diseases in disease control and prevention.On January 18,2020,the 2019-nCoV nucleic acid detection test was listed as the diagnostic criteria in the"guidelines for diagnosis and treatment of pneumonia due to 2019-nCoV(Trial Version 2)".Therefore,standardizing the operation process of the 2019-nCoV nucleic acid detection in clinical laboratories has become a top priority.It is of paramount importance to establish standard protocols for detection of the 2019-nCoV nucleic acids in clinical laboratories to improve the reliability of the results and ensure the biosafety of laboratory personnel.

关 键 词：冠状病毒属 RNA 病毒 聚合酶链反应 实验室 医院 医学检验科学 规范 控制生物危害物扩散 

分 类 号：R4[医药卫生—临床医学] R5