新型冠状病毒核酸检测假阴性结果原因分析及对策  

Causes and countermeasures of false-negative results for 2019 novel coronavirus nucleic acid test

作　　者：里进[1] 叶光明[1] 陈良君 王嘉俊 李一荣[1] Li Jin;Ye Guangming;Chen Liangjun;Wang Jiajun;Li Yirong(Department of Laboratory Medicine,Zhongnan Hospital,Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院检验科,武汉430071

出　　处：《中华检验医学杂志》2020年第3期221-225,共5页Chinese Journal of Laboratory Medicine

基　　金：战略性国际科技创新合作重点专项,国际新发病毒关键毒力因子的发现及生物学基础研究(2018YFE0204502)。

摘　　要：2019年12月以来武汉市发现多起不明原因的病毒性肺炎病例,后经研究证实引起该肺炎的病毒是以前从未在人体中发现的新型病毒,世界卫生组织将该病毒命名为2019新型冠状病毒(2019-nCoV)。2019-nCov的核酸检测是新型冠状病毒肺炎确诊的必要指标之一,但最近中日友好医院出现的"三阴"病例,以及杭州报道1例7次核酸检测前6次均为阴性的病例,导致临床医生对2019-nCov核酸检测的价值产生了很大的质疑。本文对武汉大学中南医院已开展的3000多例2019-nCov核酸检测结果进行梳理,深入分析假阴性结果产生的可能原因,并探讨其解决对策,呼吁公众理性认识核酸检测。In December 2019,a cluster of patients with pneumonia of unknown cause were linked to a seafood wholesale market in Wuhan,China.Some studies found that the virus was a new kind of virus which had never been found in the human body.Then,the virus was named 2019 novel coronavirus(2019-nCoV)by the World Health Organization(WHO).2019-nCoV nucleic acid detection is one of the essential indicators of COVID-19.Recently,some false-negative cases in China-Japan Friendship Hospital and Hangzhou Hospital led the clinical doctors to question the value of the nucleic acid detection.In this paper,more than 3000 results of 2019-nCoV detection in Zhongnan Hospital,Wuhan University were analyzed.Attention should be paid to the root cause of false-negative results and the related countermeasures should be taken.

关 键 词：冠状病毒属 核酸类 聚合酶链反应 假阴性反应 质量控制 

分 类 号：R4[医药卫生—临床医学] R5