光学相干层析显微成像的技术与应用  

Optical Coherence Microscopy and Its Application

作　　者：韩涛[1] 邱建榕 王迪[1] 孟佳 刘智毅 丁志华[1] Han Tao;Qiu Jianrong;Wang Di;Meng Jia;Liu Zhiyi;Ding Zhihua(State Key Laboratory of Modern Optical Instrumentation,College of Optical Science and Engineering,Zhejiang University,Hangzhou,Zhejiang 310027,China)

机构地区：[1]浙江大学光电科学与工程学院现代光学仪器国家重点实验室,浙江杭州310027

出　　处：《中国激光》2020年第2期56-72,共17页Chinese Journal of Lasers

基　　金：国家重点研发计划(2017YFA0700501);国家自然科学基金(11974310,61905214,31927801);浙江省自然科学基金(LR20F050001);中央高校基本科研业务费专项资金(2019QNA5004)。

摘　　要：光学相干层析显微成像(OCM)技术是一种使用相干探测的光学显微成像技术。OCM不仅具有光学相干层析成像技术(OCT)高轴向分辨、高信噪比、无需标记的优势,而且能通过高倍物镜获得高横向分辨能力,能实现微米量级的空间分辨率。首先介绍OCM技术的基本原理和实现方案,然后详细阐述OCM技术的原理以及在国际上的研究进展。针对OCM技术中如何实现超高分辨成像、焦深限制成像深度等问题,对目前该研究领域一些先进的OCM技术进行总结。OCM技术在生物医学、材料检测等领域具有广泛的应用前景。Optical coherence microscopy(OCM)utilizes a coherent detection method for optical microscopy.Having advantages,such as high axial resolution,high signal-to-noise ratio,and label-free imaging in optical coherence tomography(OCT),the OCM can achieve a micron-scale spatial resolution by utilizing a high-power objective for obtaining high lateral resolution.Initially,the basic principle and implementation scheme of the OCM technology are introduced;subsequently,the principles and research progress of the OCM technology around the world are summarized.This study also examines some advanced OCM technologies considering the problems that how to realize ultra-high-resolution imaging and that the depth of focus limits the imaging depth.The OCM technology has broad application prospect in biomedicine,material detection,and other fields.

关 键 词：医用光学 光学相干层析显微成像 生物医学成像 共聚焦显微成像 超高分辨率 深层组织成像 

分 类 号：R318.51[医药卫生—生物医学工程]