整体器官的光透明成像方法综述  

Review of Tissue Optical Clearing Methods for Imaging Whole Organs

作　　者：俞婷婷[1,2] 朱 Yu Tingting;Zhu Dan(Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology,Britton Chance Center for Biomedical Photonics,Wuhan,Hubei 430074,China;MoE Key Laboratory for Biomedical Photonics,Huazhong University of Science and Technology,Wuhan,Hubei 430074,China)

机构地区：[1]华中科技大学-武汉光电国家研究中心,Britton Chance生物医学光子学研究中心,湖北武汉430074 [2]华中科技大学生物医学光子学教育部重点实验室,湖北武汉430074

出　　处：《中国激光》2020年第2期95-111,共17页Chinese Journal of Lasers

基　　金：国家自然科学基金(81701354,31571002,61860206009,81870934,61721092);国家重点研发计划项目(2017YFA0700501);武汉光电国家研究中心创新专项、东南大学生物电子学国家重点实验室开放研究基金。

摘　　要：现代光学成像技术与荧光标记技术不断发展,为高分辨地获取生物组织三维结构信息提供了重要的工具。然而,大多数生物组织具有不透明特性,限制了光在组织中的穿透深度,进而限制了光学成像技术在大组织或整体器官成像中的应用。近年兴起的组织光透明技术通过多种物理、化学手段降低组织对光的衰减,增加光穿透深度,从而提高光学成像的成像深度与成像质量,为整体组织器官的三维成像提供了全新的思路。本文从离体组织光透明方法、大组织器官标记方法、三维整体成像技术三个方面,对整体器官的光透明成像方法进行综述。The technological development in modern optical imaging and fluorescent labeling has provided important tools for obtaining high-resolution three-dimensional(3 D)structural information about biological tissues.However,the opaque nature of most biological tissues limits the light penetration depth,which limits their applications for large tissue specimens or organs.In recent years,optical clearing methods that employ various physical and chemical means have been proposed for reducing light attenuation and improving the imaging depth,thereby providing novel perspectives for 3 D imaging of large samples or whole organs.This paper reviews the tissue optical clearing methods for imaging whole organs from three aspects:in vitro optical clearing methods for tissues,whole-mount labeling methods,and 3 D imaging techniques.

关 键 词：生物光学 三维成像 组织光透明 整体器官 光学成像 大组织标记 

分 类 号：Q632[生物学—生物物理学]