基于交替下降条件梯度的低光子数荧光寿命分析  

Low Photon Count Fluorescence Lifetime Analysis Based on Alternating Descent Conditional Gradient Method

作　　者：张潇 林丹樱[1] 牛敬敬 刘雄波 张娇 于斌[1] 张炜 屈军乐[1] Zhang Xiao;Lin Danying;Niu Jingjing;Liu Xiongbo;Zhang Jiao;Yu Bin;Zhang Wei;Qu Junle(Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen,Guangdong 518060,China)

机构地区：[1]深圳大学物理与光电工程学院光电子器件与系统(教育部/广东省)重点实验室,广东深圳518060

出　　处：《中国激光》2020年第2期280-286,共7页Chinese Journal of Lasers

基　　金：国家重点研发计划(2017YFA0700500);国家自然科学基金(61775144,61975131,61525503);广东省高等学校科技创新及团队项目(2015KGJHZ002,2016KCXTD007,2014A030312008);深圳市基础研究项目(JCYJ20170818144012025,JCYJ20170818141701667)。

摘　　要：发展适用于低光子数应用环境的荧光寿命分析方法对快速荧光寿命显微成像(FLIM)方法的发展和应用都具有重要意义。受高密度单分子定位显微成像中压缩感知算法的启发,将荧光寿命分析看成一个稀疏逆问题,提出了一种基于交替下降条件梯度(ADCG)的荧光寿命分析新方法,并通过对模拟数据和实验数据进行分析,验证了ADCG-FLIM算法即使在低光子数情形下依然能够较好地分析荧光寿命,从而有利于活细胞快速FLIM技术的发展和应用。The development of fluorescence lifetime analysis method suitable for low photon count is of great significance for the development and application of fast fluorescence lifetime imaging microscopy(FLIM).In this paper,we consider the fluorescence lifetime analysis,inspired by the compression sensing algorithm in high-density single molecule localization microscopy,as a sparse inverse problem,and we propose an alternating descent conditional gradient(ADCG)based method for fluorescence lifetime analysis.Through the analysis of simulation data and experimental data,we demonstrate that the ADCG-FLIM algorithm can be appropriately implemented to analyze fluorescence lifetime even in the case of low photon count,thereby benefiting the development and application of live cell fast FLIM.

关 键 词：生物光学 荧光寿命显微成像 时间相关单光子计数 低光子数 荧光寿命分析 交替下降条件梯度 

分 类 号：O436[机械工程—光学工程]