0.1 THz辐射对大鼠海马神经元兴奋性的影响  

Effect of 0.1 THz Radiation on Excitability of Hippocampal Neurons in Sprague Dawley Rats

作　　者：张欣欣 何明霞[1,2,3] 赵晋武[1,2,3] 陈勰宇 刘立媛 卢晓云 田甜[1,2,3] 陈孟秋 王璞[1,2,3] Zhang Xinxin;He Mingxia;Zhao Jinwu;Chen Xieyu;Liu Liyuan;Lu Xiaoyun;Tian Tian;Chen Mengqiu;Wang Pu(School of Precision Instrument&Opto-Electronics Engineering,Tianjin University,Tianjin 300072,China;State Key Laboratory of Precision Measurement Technology and Instruments,Tianjin University,Tianjin 300072,China;Terahertz Research Center,Tianjin University,Tianjin 300072,China;College of Life Science and Technology,Xi′an Jiaotong University,Xi′an,Shaanxi 710048,China)

机构地区：[1]天津大学精密仪器与光电子工程学院,天津300072 [2]天津大学精密测试技术及仪器国家重点实验室,天津300072 [3]天津大学太赫兹研究中心,天津300072 [4]西安交通大学生命科学与技术学院,陕西西安710048

出　　处：《中国激光》2020年第2期287-293,共7页Chinese Journal of Lasers

基　　金：国家自然科学基金(61675151)。

摘　　要：采用频率为0.1 THz、功率密度为2.65 mW/cm2的太赫兹光源分别辐射SD大鼠海马神经元5,15,25 min,通过神经元膜电位的变化,研究了太赫兹辐射对海马神经元兴奋性的影响,结果发现,15 min和25 min的太赫兹辐射会显著诱发海马神经元去极化,从而提高其兴奋性。为了探究太赫兹辐射提高神经元兴奋性的原因,检测了神经元内Ca^2+、Na^+和K+浓度的变化,结果表明,此辐射使海马神经元内Ca^2+、Na^+浓度增加,K+浓度减小。研究证实了太赫兹辐射(0.1 THz,2.65 mW/cm^2)通过调节海马神经元内带电离子的浓度促使其兴奋,为太赫兹辐射技术在生物医学领域应用的发展奠定了前期实验基础。In this work, the effect of terahertz(THz) radiation on the excitability of hippocampal neurons is studied by changing the neuronal membrane potential and using hippocampal neurons in Sprague Dawley(SD) rats irradiated by a THz source with frequency of 0.1 THz and power density of 2.65 mW/cm2 for 5, 15, and 25 min, respectively. The results show that THz irradiation for 15 and 25 min causes a significant depolarization of the hippocampal neurons, thereby increasing neuron excitability. To explore the mechanism behind this THz radiation-induced excitability of neurons, the intracellular concentrations of Ca^2+, Na^+, and K^+ are determined. The results show that Ca^2+ and Na^+ concentrations in the hippocampal neurons increase and K^+ concentration decreases after irradiation by the THz source. Our study shows that the irradiation with frequency of 0.1 THz and power density of 2.65 mW/cm^2 can promote neuronal excitation by regulating the concentration of charged ions in the hippocampal neurons. This finding may provide the preliminary experimental basis for the application of THz radiation technology to the biomedical field.

关 键 词：生物医学 太赫兹辐射 海马神经元 荧光检测 兴奋 离子浓度 

分 类 号：Q632[生物学—生物物理学]