基于SERS光谱技术的甲胎蛋白超灵敏定量检测  

Ultrasensitive Quantitative Detection of Alpha-Fetoprotein Based on SERS Spectroscopy

作　　者：王廷银 王运燚 林学亮 许云超 林惠晶 刘晓坤 冯尚源 Wang Tingyin;Wang Yunyi;Lin Xueliang;Xu Yunchao;Lin Huijing;Liu Xiaokun;Feng Shangyuan(College of Photonic and Electronic Engineering,Fujian Normal University,Fuzhou,Fujian350007,China;Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education,Fuzhou,Fujian350007,China)

机构地区：[1]福建师范大学光电与信息工程学院,福建福州350007 [2]医学光电科学与技术教育部重点实验室,福建福州350007

出　　处：《中国激光》2020年第2期309-316,共8页Chinese Journal of Lasers

基　　金：国家自然科学基金(61975031,U1605253,U1805263,81741008);中国教育部创新团队发展计划(IRT15R10);福建省自然科学基金(2018J01786);福建省引导性项目(2019H0009)。

摘　　要：将二维表面增强拉曼(SERS)增强基底与甲胎蛋白适配体相结合,开展甲胎蛋白(AFP)超灵敏定量检测。通过修饰有甲胎蛋白适配体的二维SERS基底和修饰有适配体互补序列的银纳米粒子间的碱基互补配对耦联来构筑纳米间隙SERS"热点"。该适配体互补序列修饰有拉曼信号标记分子ROX,AFP的加入会破坏这种纳米间隙"热点"结构,导致标记分子SERS信号强度减弱,根据ROX的SERS光谱信号变化工作曲线实现对AFP的超灵敏定量检测。这种检测方法的检测限为145 fg/mL,较传统临床检测方法提高了一个数量级,此外,设计的这种AFP SERS探针具有良好的特异性与抗干扰性能。研究结果表明提出的新型检测方法很有可能为准确检测AFP提供一种快速、有效的分析手段。In this paper,the ultrasensitive detection of an alpha-fetoprotein(AFP)is performed using a combination of two-dimensional(2 D)surface-enhanced Raman scattering(SERS)and an AFP aptamer.We construct SERS"hot spots"through complementary base-pairing between substrates modified with AFP aptamer and silver nanoparticles modified with aptamer complementary sequence.The complementary adaptor sequence is also modified using Raman signal labeled molecule ROX,but the addition of the AFP damages the structure of nano-gap"hot spots",resulting in a decrease in intensity of the SERS signal.The ultrasensitive quantitative detection of AFP is realized using the changed working curve of the SERS signal of ROX.The detection limit of this method is 145 fg/mL,which is one order of magnitude higher than that of the traditional clinical detection method.The designed AFP SERS probe also exhibits good specificity and anti-interference ability.These results show that this new approach may provide a rapid and effective method for the accurate detection of AFP.

关 键 词：生物光学 甲胎蛋白 适配体 表面增强拉曼光谱 超灵敏检测 

分 类 号：R446[医药卫生—诊断学] O657.37[医药卫生—临床医学]