回音壁模式光学微腔识别细胞类型  

Identifying Single Cell Types via Whispering Gallery Mode Optical Microcavities

作　　者：王亚平 王秀翃 王璞[1] Wang Yaping;Wang Xiuhong;Wang Pu(Beijing Engineering Research Center of Laser Technology,Key Laboratory of Trans-Scale Laser Manufacturing Technology,Ministry of Education China,Institute of Laser Engineering,Beijing University of Technology,Beijing100124)

机构地区：[1]北京市激光应用技术工程技术研究中心,跨尺度激光成型制造技术教育部重点实验室,北京工业大学激光工程研究院,北京100124

出　　处：《中国激光》2020年第2期325-331,共7页Chinese Journal of Lasers

基　　金：国家自然科学基金(61527822);国家重点研发计划(2017YFB0405201);北京市自然科学基金(4192013,L182011)。

摘　　要：回音壁模式是光子在一个准二维平面内运动,并不断地在微腔边界发生全反射而不折射出腔的一种光学模式,具有高的Q值和小的模式体积,对外部环境的变化极其敏感。利用回音壁模式可以使宽带荧光实现窄光谱的激光输出。利用掺杂DG(dragon green)荧光染料的聚苯乙烯微球作为回音壁模式光学微腔,通过细胞的吞噬功能,使荧光微球到达细胞内部,利用纳秒脉冲激光进行泵浦,实现了细胞内的回音壁模式激光输出。与在纯水环境中的激光输出相比,细胞内荧光微球回音壁模式的谐振峰发生了红移,且红移量与细胞类型有关,说明可以用回音壁模式实现细胞种类的无标记识别。Whispering gallery mode is a type of optical mode where photons move in a quasi-two-dimensional plane,and the total reflection occurs at the boundary of the microcavity without reflecting out of the cavity.This mode has a high Q value and small mode volume,and it is extremely sensitive to changes in the surrounding environment.A broadband fluorescence can be transformed into narrow-spectrum laser output by using the whispering gallery mode.In this paper,polystyrene microspheres doped with the dragon green fluorescent dye are used as whispering gallery mode optical microcavity.Through the phagocytosis of cells,the fluorescent microspheres reach inside cells and then are pumped by nanosecond pulsed laser to achieve the output of whispering gallery mode laser in cells.In comparison with the laser output in the pure-water environment,a redshift of the intracellular fluorescent microsphere whispering gallery mode resonance emission can be observed,and the redshift is related to cell type;therefore,it can be used for unlabeled identification of cell type.

关 键 词：生物光学 回音壁模式 微球腔 细胞内吞 生物传感 波长偏移 

分 类 号：O437[机械工程—光学工程]