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作 者:赵春程 李晓宁 张寅坤 张婷 傅金民 ZHAO Chuncheng;LI Xiaoning;ZHANG Yinkun;ZHANG Ting;FU Jinmin(Coastal Salinity Tolerant Grass Engineering and Technology Research Center Resource and Environmental Engineering College/Ludong University,Shandong Province,Yantai 264000,Shandong,China)
机构地区:[1]山东省滨海耐盐草业工程技术研究中心/鲁东大学资源与环境工程学院,山东烟台264000
出 处:《草业科学》2020年第4期669-677,共9页Pratacultural Science
基 金:国家自然科学基金面上项目“基于CRISPR/Cas 9技术解析多年生黑麦草耐盐MATE基因分子调控网络”(31772662)。
摘 要:为研究不同品种多年生黑麦草(Lolium perenne)的抗旱能力,选取4个多年生黑麦草品种,采用水培试验的方法,在温室利用PEG-6000模拟干旱胁迫,测定多年生黑麦草的草坪质量、地上部与地下部增长量、含水量、叶绿素含量以及抗氧化酶活性等生理指标。研究表明,干旱胁迫至10 d时,4个多年生黑麦草品种生长均受到明显抑制,同时4个品种的植株含水量都处于下降的趋势。此外,4个品种中超氧化物歧化酶、过氧化物酶、过氧化氢酶等抗氧化酶活性都出现了先上升后下降的趋势,丙二醛含量增加。利用隶属函数分析法进行抗旱性评价。结果表明,4种多年生黑麦草的抗旱能力强弱表现为Pr521>维达斯>百灵鸟>Pr525。In order to study the drought resistance of different varieties of perennial ryegrass(Lolium perenne),four perennial ryegrass varieties were selected,and turf quality was determined by hydroponic experiment in the greenhouse using Macrogol 6000(PEG-6000)to simulate drought stress.The physiological indexes of perennial ryegrass were determined,such as turf quality,aerial growth and root growth,water content,chlorophyll content and antioxidant enzyme activity.Results showed that after ten days of drought stress,the growth of the four perennial ryegrass varieties was obviously inhibited,and the water content of the four varieties was decreasing.In addition,the activity of antioxidant enzymes such as superoxide dismutase,peroxidase and catalase in the four varieties initially increased,and then decreased,and the content of malondialdehyde increased.Ranking of drought resistance was carried out using membership function analysis.The results indicated that the drought resistance of the four perennial ryegrass varieties ranked as:Pr521>Vidas>Larking>Pr525.
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