反刍动物食糜肽氮含量测定方法  

Determination of peptide nitrogen in ruminant digesta

作　　者：李妙善 王虎成[1] 张霞[1] LI Miaoshan;WANG Hucheng;ZHANG Xia(Experimental Teaching Center of Pratacultural Science/College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,Gansu,China)

机构地区：[1]兰州大学草业科学实验教学中心/兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2020年第4期784-790,共7页Pratacultural Science

基　　金：国家重点研发计划(2018YFD0502302)。

摘　　要：以牛的瘤胃和皱胃食糜为材料,采用L9(34)正交试验设计,研究Tris-HCl缓冲液浓度(0.02、0.04和0.06 mol·L^–1)、pH(6.8、7.4和8.0)、料液比(1∶15、1∶20和1∶25)及浸提时间(40、60和80 min)对牛胃食糜内肽氮含量测定结果的影响,旨在优选出最佳的肽氮测定方法。结果表明,牛胃食糜氮素分离条件为Tris-HCl缓冲液浓度0.06 mol·L^–1、pH 6.8、料液比1∶25、浸提60 min时,所测定的肽氮含量最大值为14.97 mg·g^–1(瘤胃食糜)和19.28 mg·g^–1(皱胃食糜)。In the present experiment,the cattle digesta from the rumen and abomasum were used to establish a moderate method for the determination of the peptide nitrogen content of the ruminant gastrointestinal digesta using the L9(34)orthogonal design.The effects of the Tris-HCl buffer concentration(0.02,0.04,or 0.06 mol·L^–1),Tris-HCl buffer pH(6.8,7.4,or 8.0),ratio of dry digesta to Tris-HCl buffer(1∶15,1∶20,or 1∶25),and extraction time(40,60,or 80 min)on the determination of the peptide nitrogen content in the cattle gastric digesta were studied.The results show that the moderate separation conditions of nitrogen from cattle gastric digesta were 0.06 mol·L^–1,6.8,1∶25,and 60 min,respectively,and the maximum contents of peptide nitrogen were 14.97 mg·g^–1 and 19.28 mg·g^–1 in the rumen and abomasum digesta,respectively.

关 键 词：牛 食糜 肽氮含量 测定方法 

分 类 号：S816[农业科学—饲料科学]