球孢白僵菌固定化生物转化合成D-HPPA  

Biotransformation of D-PPA to D-HPPA by immobilized Beauveria bassiana

作　　者：王灿 郝会会 潘海亮 赵筱[1] 王金华[1] WANG Can;HAO Hui-hui;PAN Hai-liang;ZHAO Xiao;WANG Jin-hua(Key Laboratory of Fermentation Engineering of Ministry of Education,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学生物与食品学院,发酵工程教育部重点实验室,湖北武汉430068

出　　处：《生物技术》2020年第1期98-103,共6页Biotechnology

基　　金：国家“十二五”支撑计划项目(2012BAD27B03);湖北工业大学博士科研启动基金项目(BSQD12143)。

摘　　要：[目的]利用球孢白僵菌进行固定化生物转化,将底物R-(+)-2-苯氧基丙酸(D-PPA)转化合成产物R-(+)-2-(4-羟基苯氧基)丙酸(D-HPPA)。[方法]利用海藻酸钠和聚乙烯醇对球孢白僵菌进行包埋处理,并对包埋条件进行累积优化。[结果]4%海藻酸钠和4.5%聚乙烯醇混合后,再加入2.5%的氯化钙作为交联剂交联8 h。在此包埋条件下制备的白僵菌凝胶珠,置于30 g/L的D-PPA进行固定化生物转化。反应5 d后,产物浓度最终为29.9 g/L,平均生产强度为5.98 g/(L·d),底物转化率为99.7%。[结论]海藻酸钠和聚乙烯醇可用于白僵菌的固定化,且较游离菌体的生物转化的反应周期缩短28.6%,平均生产强度增加64.7%,底物转化率提高17.7%。[Objective]Beauveria bassiana was used for immobilized biotransformation and the conversion of R-(+)-2-phenoxypropionic acid(D-PPA)into R-(+)-2-(4-Hydroxyphenoxy)propionic acid(D-HPPA).[Method]The use of sodium alginate and polyvinyl alcohol for embedding of Beauveria bassiana was carried out and the embedding conditions were cumulatively optimized.[Result]After mixing with 4%sodium alginate and 4.5%polyvinyl alcohol,2.5%calcium chloride was then added in as a cross-linking agent for 8 hours for Beauveria bassiana embedding.The generated gel beads were placed in 30 g/L D-PPA for immobilized biotransformation.After 5 days of reaction,the final product concentration was 29.9 g/L,while the average production strength was 5.98 g/(L·d).The substrate conversion rate was 99.7%.[Conclusion]Sodium alginate and polyvinyl alcohol can be used for the immobilization of Beauveria bassiana and improve the performance of biotransformation.The reaction cycle of biotransformation is shortened by 28.6%.The average production intensity is increased by64.7%,and the substrate conversion rate is increased by 17.7%.

关 键 词：球孢白僵菌 R-(+)-2-(4-羟基苯氧基)丙酸 生物转化 固定化 

分 类 号：Q815[生物学—生物工程]