核酸反应性标本9例与对应ELISA、CLIA法检测HBV的关联分析  

Analysis of the relationship between 9 cases of nucleic acid reactive samples and the detection of HBV by ELISA and CLIA

作　　者：范菲 Fan Fei(Changsha Blood Center,Hunan Changsha 410005)

机构地区：[1]长沙血液中心,湖南长沙410005

出　　处：《中国社区医师》2020年第13期114-115,共2页Chinese Community Doctors

摘　　要：目的:分析核酸反应性标本和对应ELISA、CLIA法检测HBV的关联性。方法:选取2017年1月-2018年1月无偿献血者1000例,提供核酸检测,同时对核酸反应性标本提供CLIA法检测。结果:通过核酸检测出HBV DNA反应性标本9例,没有检测出HCV RNA及HIV RNA反应性标本,其中属于ELISA法阴性高值标本1例;9例核酸反应性标本对应CLIA法HBV两对半检测产生HBsAg(-)抗-HBc(+)2例,HBsAg(-)抗-HBc(+)抗-HBe(+)血清型6例,剩余无反应性1例,怀疑为窗口期标本。结论:对于无偿献血者血液开展核酸检测以及ELISA法检测存在互补性,核酸检测能够良好弥补ELISA法检测窗口期问题,核酸检测和ELISA法检测存在相同结果,将影响血液质量的ELISA法阴性高值标本淘汰,能够有效减少输血感染的出现风险。Objective:To explore the relationship between nucleic acid reactive samples and the detection of HBV by ELISA and CLIA.Methods:1000 unpaid blood donors were selected,who were tested for nucleic acid and provided CLIA test for nucleic acid reactive samples.Results:9 HBV DNA reactive samples were detected by nucleic acid,but no HCV RNA and HIV RNA reactive samples were detected,one case was negative high value specimen by ELISA;In 9 cases of nucleic acid reactive samples,corresponding to the two-and-a-half detection of HBV by CLIA method,2 cases were HBsAg(-)anti-HBC(+)and 6 cases were HBsAg(-)anti-HBc(+)anti-HBe(+)serotypes,one case remained unresponsive,it was suspected to be a window specimen.Conclusion:There is complementarity between NAT test and ELISA test on blood of unpaid blood donors,NAT detection can make up for the problem of detection window period of ELISA,and NAT and ELISA have the same results,the elimination of negative and high value samples of ELISA that affect blood quality can effectively reduce the risk of transfusion infection.

关 键 词：核酸反应性标本 ELISA法 CLIA法 HBV 关联性 

分 类 号：R446.6[医药卫生—诊断学] R512.62[医药卫生—临床医学]