对2011~2015年达州市5478份HIV抗体初筛阳性的血液标本进行复检和确证试验情况的分析  

The analysis of the reexamination and confirmation test of 5478 blood samples with HIV antibody positive primary screening in dazhou city from 2011 to 2015

作　　者：罗小莉 王顺东[1] 林忠荔 张静 王芸 王开宇[1] Luo xiaoli;Wang shundong;Lin zhongli;Zhang jing;Wang yun;Wang kaiyu(sichuan provincial center for disease control and prevention,dazhou 635000,China)

机构地区：[1]四川省达州市疾病预防控制中心,达州635000

出　　处：《当代医药论丛》2020年第5期6-8,共3页

摘　　要：目的:分析对2011~2015年达州市5478份人类免疫缺陷病毒(HIV)抗体初筛结果呈阳性的血液标本进行复检和确证试验的情况。方法:将2011年1月至2015年12月在达州市进行HIV抗体初筛结果呈阳性的5478份血液标本作为研究对象。对这些血液标本进行HIV抗体复检,对复检结果呈阳性的血液标本进行HIV抗体确证试验,然后对复检和确证试验的结果进行统计分析。结果:对5478份HIV抗体初筛结果呈阳性的血液标本进行HIV抗体复检结果的阳性率为62.19%(3407份)。对3407份HIV抗体复检结果呈阳性的血液标本进行HIV抗体确证试验结果的阳性率为92.81%(3162份)。在3162份HIV抗体确证试验结果呈阳性的血液标本中,来自于疾控中心血液标本的占比为67.77%,来自于自愿咨询检测人员血液标本的占比为45.00%,检测结果中出现9条带的占比为43.67%,条带gp160、gp120、gp41、p66、p51、p31、p55、p24及p17的阳性率分别为100%、100%、97.43%、66.70%、64.10%、91.05%、51.64%、96.46%及64.48%。在122份HIV抗体确证试验结果为“不确定”的血液标本中,检测结果中单一条带p24、单一条带gp160的阳性率分别为27.87%、21.31%。在30份HIV抗体确证试验结果为“不确定”但后期转为阳性的血液标本中,第一次确证试验结果中出现条带gp160的标本阳转率最高,占比66.67%,其次为第一次确证试验结果中出现条带p24的标本,占比为46.67%。结论:2011~2015年达州市进行HIV抗体确证试验结果呈阳性的患者多为自愿咨询检测人员,血液标本的来源机构主要是疾控中心。进行HIV抗体确证试验的结果呈阳性的血液标本中,检测结果中全带呈阳性的占比最高,抗env基因编码蛋白抗体的阳性率最高。对血液标本进行HIV抗体筛查试验的结果存在假阳性,须使用免疫印迹试验对HIV抗体筛查呈阳性的血液标本进行确证试验。应对HIV抗体确证试验结果为“不确定”的标本进�objective:to analyze the reexamination and confirmatory test of 5478 blood samples of human immunodeficiency virus(HIV)antibody tested positive in the initial screening from 2011 to 2015 in dazhou city.Methods:from January 2011 to December 2015,5478 blood samples tested positive for HIV antibody were selected as subjects.The blood samples were retested for HIV antibodies,and the positive blood samples were tested for HIV antibody confirmation,followed by statistical analysis of the results of the retesting and confirmation tests.Results:the positive rate of HIV antibody reexamination was 62.19%(3407 samples)in 5478 blood samples with HIV antibody positive primary screening.The positive rate of HIV antibody confirmation test was 92.81%(3162)in 3407 blood samples that were retested positive for HIV antibody.Among the 3162 blood samples with positive HIV antibody test results,the proportion of blood samples from the centers for disease control and prevention(CDC)was 67.77%,and the proportion of blood samples from volunteers was 45.00%,and the proportion of 9 bands in the test results was 43.67%.The positive rates of bands gp160,gp120,gp41,p66,p51,p31,p55,p24 and p17 were 100%,100%,97.43%,66.70%,64.10%,91.05%,51.64%,96.46%and 64.48%,respectively.Among the 122 blood samples with"uncertain"HIV antibody confirmation test results,the positive rates of single band p24 and single band gp160 were 27.87%and 21.31%,respectively.Among the 30 blood samples whose HIV antibody test results were"uncertain"but turned positive later,those with band gp160 in the first test results had the highest positive conversion rate(66.67%),followed by those with band p24 in the first test results(46.67%).Conclusion:from 2011 to 2015,positive HIV antibody test results of patients in dazhou city are mostly voluntary counseling testing personnel,the source of blood samples is mainly the center for disease control and prevention.Among the blood samples tested positive for HIV antibody,the proportion of all positive blood samples was the highest,and

关 键 词：人类免疫缺陷病毒 HIV抗体检测 确证试验 

分 类 号：R446[医药卫生—诊断学]