黄芪和天花粉对小鼠恶性黑色素瘤B16细胞增殖和迁移及E-cadherin、N-cadherin蛋白表达的影响  

Effects of Astragali Radix and Trichosanthis Radix on Cell Proliferation and Migration of Mouse Malignant Melanoma B16 Cells and Expressions of E-cadherin and N-cadherin

作　　者：张秋艳 黄松丽 张琳婧 梁羽茜 赵丕文[1] 魏胜利[1] 胡秀华[1] ZHANG Qiuyan;HUANG Songli;ZHANG Linjing;LIANG Yuxi;ZHAO Piwen;WEI Shengli;HU Xiuhua(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中医药信息杂志》2020年第5期28-34,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81673764);北京中医药大学校级自主课题(2017-JYB-XS-032);子洲黄芪规范化种植新技术开发研究(2020071720274)。

摘　　要：目的观察黄芪、天花粉及其配伍对小鼠恶性黑色素瘤B16细胞增殖和迁移的影响,探讨其作用机制。方法 MTT法检测B16细胞增殖,根据结果计算细胞生长抑制率为0%时对应的各药物浓度(IC0)、为25%时对应的各药物浓度(IC25)、为50%时对应的各药物浓度(IC50)。设空白组、配伍组(黄芪注射液IC0+天花粉水煎剂IC0、黄芪注射液IC0+天花粉水煎剂IC25、黄芪注射液IC0+天花粉水煎剂IC50、黄芪注射液IC25+天花粉水煎剂IC0、黄芪注射液IC25+天花粉水煎剂IC25)。细胞划痕实验检测B16细胞迁移,Westernblot检测B16细胞迁移相关蛋白E-cadherin、N-cadherin表达。结果 MTT检测结果表明,不同剂量黄芪和天花粉均可抑制B16细胞增殖,具有浓度依赖性;细胞划痕实验结果显示,黄芪和天花粉均影响B16细胞划痕愈合,抑制细胞迁移,具有浓度依赖性;两药不同比例配伍的细胞增殖抑制率明显高于单味中药,差异有统计学意义(P<0.05);Westernblot检测结果显示,黄芪和天花粉均增加E-cadherin蛋白的表达,降低N-cadherin蛋白的表达。结论黄芪、天花粉及其配伍均可抑制小鼠恶性黑色素瘤B16细胞增殖及迁移,且两药配伍效果优于单味中药,黄芪和天花粉抑制细胞迁移的机制与E-cadherin和N-cadherin蛋白的表达相关。Objective To investigate the effects and mechanisms of Astragali Radix, Trichosanthis Radix and their compatibility on the proliferation and migration of mouse malignant melanoma B16 cells;To discuss its possible mechanism. Methods MTT method was used to detect the proliferation of B16 cells. Based on the results, the drug concentration corresponding to the cell growth inhibition rate of 0%(IC0), the drug concentration corresponding to 25%(IC25), and the drug concentration corresponding to 50%(IC50) were calculated. Blank group and the compatible group(Astragalus IC0 + Trichosanthin IC0, Astragalus IC0 + Trichosanthin IC25, Astragalus IC0 + Trichosanthin IC50, Astragalus IC25 + Trichosanthin IC0, Astragalus IC25 + Trichosanthin IC25) were set. Cell scratch test was used to detect B16 cell migration;Western blot was used to detect the expressions of B16 cell migration related proteins E-cadherin and N-cadherin. Results MTT results showed that Astragali Radix and Trichosanthis Radixwith different concentrations could inhibit the proliferation of B16 cells in a dose-dependent manner. Results of cell scratch test showed that both Astragali Radix and Trichosanthis Radix would affect the scratch healing of B16 cells, inhibit cell migration, and were concentration-dependent. After the compatibility of Astragali Radix and Trichosanthis Radix, the inhibition rate of cell proliferation was significantly higher than that of the single Chinese materia medica, with statistical significance(P<0.05). Western blot results showed that both Astragali Radix and Trichosanthis Radix increased the expression of E-cadherin protein and decreased the expression of N-cadherin protein. Conclusion Both Astragali Radix and Trichosanthis Radix can inhibit cell proliferation and migration of malignant melanoma B16. The compatibility of Astragali Radix and Trichosanthis Radix have better efficacy than single Chinese materia medica. Its mechanism of inhibiting cell migration may be related to the protein expressions of E-cadherin and N-cadherin.

关 键 词：黄芪 天花粉 B16细胞 细胞增殖 细胞迁移 配伍 

分 类 号：R285.5[医药卫生—中药学]