石榴SUT基因家族鉴定及其在籽粒发育过程中的表达分析  

Identification of SUT genes and their expression models during the development of seed in pomegranate

作　　者：骆翔 曹尚银[1] 李好先[1] 赵弟广 张富红[1] 陈丽娜 Krishna Poudel 敬丹 唐丽颖 LUO Xiang;CAO Shangyin;LI Haoxian;ZHAO Diguang;ZHANG Fuhong;CHEN Lina;KRISHNA Poudel;JING Dan;TANG Liying(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou,450009,Henan,China)

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009

出　　处：《果树学报》2020年第4期485-494,共10页Journal of Fruit Science

基　　金：国家自然科学基金(31901343);中国农业科学院科技创新工程(CAAS-ASTIP-2016-ZFRI-03)。

摘　　要：【目的】对石榴SUT家族基因成员进行全基因组鉴定,并分析SUT家族基因成员的结构域特征、启动子序列、生化特征、进化关系和表达特性,为深入研究该基因作用及其分子调控机制提供参考。【方法】利用同源比对及HMMER模型鉴定石榴SUT基因家族成员;利用邻近法和基因结构分析解析石榴SUT基因及其启动子序列的系统发生关系。【结果】共鉴定了10个石榴SUT基因,可以分为3大类;石榴SUT基因及其启动子区的GC含量都明显低于其在拟南芥和水稻中的含量;石榴SUT家族基因GC含量远高于启动子区的GC含量;石榴SUT基因的序列长度与GC含量呈明显正相关;石榴SUT基因启动子区含有10种MYB元件;此外,4个石榴SUT基因(PgL0328370.1、PgL0099690.1、PgL0145810.1和PgL0145770.1)在籽粒发育过程中发生了差异表达。【结论】SUT基因在进化过程中其序列和基因结构相对保守,而其启动子序列在进化过程中变化较大,SUT基因的表达量变化与籽粒发育显著相关。【Objective】Photosynthetically produced sugar,principally sucrose,is moved from source leaves to support growth of,and carbon storage by,heterotrophic sink organs.Membrane proteins play pivotal roles in mediating sucrose transport within plants.Pomegranate(Punica granatum L.),native to central Asia,is an ancient medicinal fruit crop grown worldwide,with considerable economic value.Pomegranate is well famed as a highly valuable fruit with high nutritive and medical attributes.The pomegranate’s flower,fruit peel,aril(juice),and seeds,are useful for the prevention and treatment of a wide range of diseases.Their functional advantages have dramatically stimulated the market demand,which has opened the avenue for the breeding programs in pomegranate.Mapping genes related to horticulturally important traits is helpful to the molecular marker-assisted(MAS)breeding.To data,SUTs activities had been proved to have a major impact upon regulating many plant developmental processes,such as pollen germination,flowering,tuberization,restraining plant growth,fruit size reduction,seed development,biomass partitioning,plant growth rates,crop yields and ethylene biosynthesis.Here,the objective of this study was to identify the genomic SUT genes,and analyze their gene structure,promoter,phylogenetic relationship and expression models.It will provide the reference for further research on the function and molecular regulation of SUT family in pomegranate.【Methods】Blastp and HMMER model were performed to identify the SUT genes;The phylogenetic relationships of SUTs and their promoters were analyzed by adjacent method and gene structure analysis.【Results】There were nine sucrose transporter genes described in Arabidopsis,whereas the rice genome contained five SUT genes.In the study,a total of 10 SUT genes were detected on the Chr1,Chr3,Chr4,Chr5,Chr6 and Chr7 in pomegranate,whose number was more than that of Arabidopsis and rice.The average sequence length of SUT genes was 4406.7 bp.The average content of A,C,G and T for SUT

关 键 词：石榴 SUT 基因家族 籽粒 表达分析 

分 类 号：S66[农业科学—果树学]