Metabolic profiling of four synthetic stimulants,including the novel indanyl-cathinone 5-PPDi,after human hepatocyte incubation  

作　　者：David Fabregat-Safont Marie Mardal Juan V.Sancho Félix Hernández Kristian Linnet María Ibánez 

机构地区：[1]Research Institute for Pesticides and Water,University Jaume I,Avda.Sos Baynat s/n,12071,Castellón,Spain [2]Department of Forensic Medicine,Section of Forensic Chemistry,Faculty of Health and Medicinal Sciences,University of Copenhagen,Frederik V's vej 11,2100,KФbenhavnФ,Denmark [3]Department of Occupational and Environmental Medicine,University Hospital of Northern Norway,Sykehusvegen,Tromsoe,Norway

出　　处：《Journal of Pharmaceutical Analysis》2020年第2期147-156,共10页药物分析学报（英文版）

基　　金：financial support from the Ministerio de Economía y Competitividad Spain(Project CTQ2015-65603-P);from Universitat Jaume I(UJI-B2018-19);Ministerio de Educación,Cultura y Deporte in Spain for his predoctoral grant(FPU15/02033);financial support received for his research stay at the University of Copenhagen(EST17/00024)。

摘　　要：Synthetic cathinones are new psychoactive substances that represent a health risk worldwide.For most of the 130 reported compounds,information about toxicology and/or metabolism is not available,which hampers their detection(and subsequent medical treatment)in intoxication cases.The principles of forensic analytical chemistry and the use of powerful analytical techniques are indispensable for stab-lishing the most appropriate biomarkers for these substances.Human metabolic fate of synthetic cathinones can be assessed by the analysis of urine and blood obtained from authentic consumers;however,this type of samples is limited and difficult to access.In this work,the metabolic behaviour of three synthetic cathinones(4-CEC,4-CPrC and 5-PPDi)and one amphetamine(3-FEA)has been evalu-ated by incubation with pooled human hepatocytes and metabolite identification has been performed by high-resolution mass spectrometry.This in vitro approach has previously shown its feasibility for obtaining excretory human metabolites.4-CEC and 3-FEA were not metabolised,and for 4-CPrC only two minor metabolites were obtained.On the contrary,for the recently reported 5-PPDi,twelve phase I metabolites were elucidated.Up to our knowledge,this is the first metabolic study of an indanyl-cathinone.Data reported in this paper will allow the detection of these synthetic stimulants in intoxi-cation cases,and will facilitate future research on the metabolic behaviour of other indanyl-based cathinones.

关 键 词：SYNTHETIC CATHINONES 5-PPDi Metabolite identification In vitro metabolism Hepatocyte INCUBATION High resolution mass spectrometry 

分 类 号：R96[医药卫生—药理学]