SERS基底Ag@Au的制备及其对氧氟沙星的检测  

Preparation of SERS substrate Ag@Au and its detection of ofloxacin

作　　者：程昊 徐娅娟 李利军 黄文艺 卢浩 冯军 Cheng Hao;Xu Yajuan;Li Lijun;Huang Wenyi;Lu Hao;Feng jun(Guangxi Key Laboratory of Green Processing of Sugar Resources,College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry;School of Medicine,Guangxi University of Science and Technology)

机构地区：[1]广西科技大学生物与化学工程学院,广西糖资源绿色加工重点实验室,广西柳州545006 [2]蔗糖产业省部共建协同创新中心 [3]广西科技大学医学院

出　　处：《无机盐工业》2020年第5期18-21,共4页Inorganic Chemicals Industry

基　　金：国家自然科学基金地区科学基金项目(31560466),国家自然科学基金地区项目:功能化纳米复合材料hCEs SERS探针的构建及在血清与细胞中检测羧酸酯酶活性的应用研究(81860633);广西高等学校高水平创新团队及卓越学者计划资助项目(桂教人[2014]7号);广西自然科学基金面上项目:微流控芯片-表面增强拉曼检测系统的构建及在单细胞分析中的应用(2016GXNSFAA380108);广西科技大学硕士研究生创新项目(GKYC201909)。

摘　　要：通过种子介导生长方法,合成了形貌、粒径均一,分散性良好的双金属Ag@Au纳米粒子,并将其作为表面增强拉曼散射(SERS)基底,对氧氟沙星(OFLX)进行检测。首先在最佳实验条件下对氧氟沙星主要拉曼峰进行归属,选取1416 cm-1拉曼特征峰。其次,对其1416 cm-1特征峰强度与氧氟沙星浓度作线性拟合,曲线方程为Y=291.48X+3156.8,r=0.989,检测极限可达10-10mol/L。该方法操作简单,灵敏度高,重现性好,可为氧氟沙星类抗生素药物的SERS检测提供依据。A bimetallic Ag@Au nanoparticle with uniform morphology,uniform particle size and good dispersibility was synthesized by seed-mediated growth method and used as a surface-enhanced Raman scattering(SERS) substrate for ofloxacin(OFLX) testing.Firstly,the main Raman peak of ofloxacin was assigned under the optimal experimental conditions,and the1 416 cm-1 Raman characteristic peak was selected.Secondly,the characteristic peak intensity of 1 416 cm-1 was linearly fitted with the concentration of ofloxacin.The curve equation was Y=291.48 X+3 156.8,r=0.989,and the detection limit reached10-10 mol/L.The method is simple in operation,high in sensitivity and good in reproducibility,and can provide a basis for SERS detection of ofloxacin antibiotic drugs.

关 键 词：SERS Ag@AuNPs 氧氟沙星 

分 类 号：TQ131.22[化学工程—无机化工]