社区肺炎链球菌感染药敏分析及分子生物学分型  

Antibiotic sensitivity spectrum and molecular epidemiology among Streptococcus Pneumoniae from community hospital

作　　者：向蓉 黄雪珍 莫和国 徐宁 XIANG Rong;HUANG Xuezhen;MO Heguo;XU Ning(Department of Clinical Laboratory,Xiaolan Hospital Affiliated to Southern Medical University,Zhongshan,Guangdong 528415,China)

机构地区：[1]南方医科大学附属小榄医院检验科,广东中山528415

出　　处：《检验医学与临床》2020年第9期1202-1204,共3页Laboratory Medicine and Clinic

基　　金：广东省中山市科技计划项目(2015B1077)。

摘　　要：目的调查该院肺炎链球菌药敏谱及耐药基因型,为肺炎链球菌感染的预防与治疗提供参考。方法收集该院2014年1月至2016年12月分离的肺炎链球菌共184株,用Vitek 2鉴定菌株,检测耐药模式,PCR方法检测耐药基因erm(B)、mef(A)。结果肺炎链球菌对青霉素G的耐药率为60.6%,对红霉素、克林霉素的耐药率分别为93.3%、100.0%。耐药表型为MLSB型的检出率为93.5%(cMLSB:89.7%,iMLSB:3.8%),未检出M耐药表型。耐药基因erm(B)的检出率为91.3%,mef(A)的检出率为1.6%。结论该院肺炎链球菌对红霉素与克林霉素的耐药率较高;耐药表型以erm(B)编码的cMLSB耐药表型为主,主要以靶位点的修饰为主,也与泵外排机制有关。Objective To determine the antibiotic sensitivity spectrum and resistant phenotypes of Streptococcus Pneumoniae in Xiaolan Hospital Affiliated to Southern Medical University order to provide a reasonable guidance for prevention and treatment.Methods A total of 184 Streptococcus Pneumoniae strains were collected from January 2014 to December 2016.Vitek 2 was used to identify the isolates and the resistant phenotypes.PCR was used to detect the resistant genes erm(B)and mef(A)genes.Results Penicilin G resistance was found in 60.6% of Streptococcus Pneumoniae isolated.The resistant rates of erythromycin,clindamycin were 93.3%,100.0% respectively.The detection rate of the phenotype MLSB was 93.5%(cMLSB:89.7%,iMLSB:3.8%).The phenotype M was not found.The detection rates of erm(B)gene and mef(A)gene were91.3%,1.6%respectively.Conclusion The resistant rates of Streptococcus Pneumoniae to erythromycin and clindamycin are still high.cMLSB mediated by erm(B)gene is the most predominant phenotype among macrolide-resistant Streptococcus Pneumoniae,and it is also mediated by efflux pumps.

关 键 词：肺炎链球菌 耐药基因 药敏谱 

分 类 号：R446.5[医药卫生—诊断学]