自动图像分析系统与快速流式细胞术在白细胞减少性疾病诊治中的价值  

Roles of automated image analysis system and CytoDiff flow cytometry for the diagnosis and treatment ofleucopenia

作　　者：郭平[1] 陈骊婷[1] 廖兵 王剑飚[1] GUO Ping;CHEN Liting;LIAO Bing;WANG Jianbiao(Department of Clinical Laboratory,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]上海交通大学医学院附属瑞金医院检验科,上海200025 [2]上海交通大学基础医学院,上海200025

出　　处：《检验医学》2020年第4期349-353,共5页Laboratory Medicine

基　　金：上海市2017年度“科技创新行动计划”产学研医合作领域项目(编号170Z1930301-27)。

摘　　要：目的探讨自动图像分析系统与快速流式细胞术在白细胞减少性疾病诊治中的价值。方法分别采用自动图像分析系统、快速流式细胞术和显微镜镜检法对白细胞计数为(0.5~2.0)×10^9/L的181例患者进行白细胞分类计数。以显微镜镜检法为参考方法,比较上述方法白细胞分类计数的时间、精密度以及原始细胞检测相关性、敏感性与特异性。结果显微镜镜检法、快速流式细胞术、自动图像分析系统预分类和自动图像分析系统预分类+人工审核白细胞分类计数中位时间分别为864 s、277 s、190 s、364 s;快速流式细胞术中性粒细胞、淋巴细胞、单核细胞和原始细胞分类计数结果的离散度优于其他3种方法。快速流式细胞术、自动图像分析系统预分类、自动图像分析系统预分类+人工审核检测原始细胞的敏感性和特异性分别为94.90%和98.80%、54.08%和74.69%、79.59%和100.00%,与显微镜镜检法的r值分别为0.937、0.507、0.990。结论自动图像分析系统+人工审核与快速流式细胞术可快速、准确地检测出低值白细胞样本中的原始细胞,对患者疾病的诊断、治疗及预后判断具有重要价值。Objective To evaluate the roles of automated image analysis system and CytoDiff flow cytometry for the diagnosis and treatment of leucopenia.Methods A total of 181 patients with white blood cell count(0.5-2.0)×10^9/L were enrolled and determined for differentiation and count by automated image analysis system and CytoDiff flow cytometry.Time,precision,correlation of blasts,sensitivity and specificity were compared with microscopy(standard method).Results The median times were 864,277,190 and 364 s by microscopy,CytoDiff flow cytometry,DM96 preclassification and DM96 reclassification,respectively.The variation of CytoDiff flow cytometry for neutrophils,lymphocytes,monocytes and blasts was better than those of the other 3 methods.The correlation coefficients(r)between CytoDiff flow cytometry,DM96 preclassification,DM96 reclassification and microscopy were 0.937,0.507 and 0.990,respectively.The sensitivity and specificity of CytoDiff flow cytometry were 94.90%and 98.80%,respectively.The sensitivities of DM96 preclassification and DM96 reclassification were 54.08%and 79.59%,and the specificities were 74.69%and 100.00%,respectively.Conclusions DM96 reclassification and CytoDiff flow cytometry can detect blasts in leucopenia samples with high efficiency and accuracy and meet the demand of clinic for the curative effect monitoring and prognostic analysis.

关 键 词：白细胞减少 自动图像分析系统 快速流式细胞术 

分 类 号：R446.1[医药卫生—诊断学]