Dual enzyme activated fluorescein based fluorescent probe  

作　　者：Maria L.Odyniec Jordan E.Gardiner Adam C.Sedgwick Xiao-Peng He Steven D.Bull Tony D.James 

机构地区：[1]Department of Chemistry,University of Bath,Bath,BA27AY,UK [2]Department of Chemistry,University of Texas at Austin,Austin,TX 78712-1224,USA [3]Key Laboratory for Advanced Materials&Feringa Nobel Prize Scientist Joint Research Center,East China University of Science and Technology,Shanghai 200237,China

出　　处：《Frontiers of Chemical Science and Engineering》2020年第1期117-121,共5页化学科学与工程前沿（英文版）

基　　金：the EPSRC and the University of Bath for funding.

摘　　要：A simple dual analyte fluorescein-based probe(PF3-Glc)was synthesised containingβ-glucosidase(β-glc)and hydrogen peroxide(H2O2)trigger units.The presence ofβ-glc,resulted in fragmentation of the parent molecule releasing glucose and the slightly fluorescent mono-boronate fluorescein(PF3).Subsequently,in the presence of glucose oxidase(GOx),the released glucose was catalytically converted to D-glucono-δ-lactone,which produced H2O2 as a by-product.The GOx-produced H2O2,resulted in classic H2O2-mediated boronate oxidation and the release of the highly emissive fluorophore,fluorescein.This unique cascade reaction lead to an 80-fold increase in fluorescence intensity.

关 键 词：CHEMOSENSORS dual-activation GOx fluorescence Β-GLUCOSIDASE molecular logic 

分 类 号：O62[理学—有机化学]