玉米毛状根再生植株根系的植物内源激素动态变化研究  

Dynamic research of endogenous hormone in the roots of hairy root regeneration plant of maize

作　　者：未晓巍[1,2] 陈志鹏 郭丽 刘凡语 谈韫 周晓馥[1,2] WEI Xiao-wei;CHEN Zhi-peng;GUO li;LIU Fan-yu;TAN Yun;ZHOU Xiao-fu(College of Life Science,Jilin Normal Unnversity,Siping 136000,China;Key laboratory for Plant Resources Science and Green production,Jilin Normal Unversity,Siping 136000,China)

机构地区：[1]吉林师范大学生命科学学院,吉林四平136000 [2]吉林师范大学植物资源科学与绿色生产吉林省重点实验室,吉林四平136000

出　　处：《吉林师范大学学报（自然科学版）》2020年第2期116-121,共6页Journal of Jilin Normal University:Natural Science Edition

基　　金：国家自然科学基金项目(31070224);吉林省重点科技计划项目(20120206)。

摘　　要：为了解不同时期的玉米毛状根再生植株根系生长发育与内源激素之间的关系,比较玉米毛状根再生植株与野生型材料根系的植物内源激素的含量差异,探析植物内源激素在玉米毛状根再生植株根系生长过程中的生理功能.以玉米毛状根再生植株为供试材料,采用高效液相色谱法(HPLC)分析苗期、拔节期、抽雄期、灌浆期、成熟期的根中的水杨酸(SA)、生长素(IAA)、玉米素(ZT)、赤霉素(GA3)和脱落酸(ABA)的含量.结果表明,毛状根再生植株的根系的内源激素SA、ZT、IAA显著高于野生型,GA3与ABA则反之.在玉米根系生长发育不同时期,内源激素间相互作用调控地上部植株及籽粒的形成.In order to understand the relationship between root growth and endogenous hormones in hairy root regeneration plants of maize at different periods,the content of endogenous hormones in hairy root regeneration plants of maize and wild-type material roots were compared,and the physiological functions of endogenous hormones in the root growth of hairy root regeneration plants of maize were investigated.The content of salicylic acid(SA),auxin(IAA),zein(ZT),gibberellin(GA3)and abscisic acid(ABA)in the regenerated plants with hairy roots of maize was analyzed by high performance liquid chromatography(HPLC).The results show that the endogenous hormones SA,ZT and IAA in the roots of regenerated plants with hairy roots are significantly higher than those in the wild type,while GA3 and ABA are not.SA,ZT,GA3,IAA and ABA play key regulatory roles in different stages of maize root growth and development.

关 键 词：玉米毛状根再生植株 根系 内源激素 

分 类 号：Q943.1[生物学—植物学]