腐蹄病奶牛NF-κB和p38MAPK信号通路的表达变化研究  

Expression changes of NF-κB and p38MAPK signaling pathways in dairy cow with foot rot disease

作　　者：郑家三[1] 阮红日 朱婷婷 魏雪 侯凯文 周志新 ZHENG Jiasan;RUAN Hongri;ZHU Tingting;WEI Xue;HOU Kaiwen;ZHOU Zhixin(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《黑龙江畜牧兽医》2020年第8期59-62,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家科技支撑计划项目(2012BAD12B05-2;2013BAD21B01)。

摘　　要：为了明确腐蹄病奶牛核因子κB(NF-κB)和p38丝裂原活化蛋白激酶(p38MAPK)信号通路的表达及变化情况,探讨奶牛腐蹄病炎性信号通路的作用,试验采用酶联免疫吸附试验检测腐蹄病奶牛(试验组)和健康奶牛(对照组)血清中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素4(IL-4)、p38MAPK和NF-κB的表达情况。结果表明:与对照组比,试验组奶牛血清中NF-κB和IL-1β含量显著升高(P<0.05);IL-4含量极显著升高(P<0.01);TNF-α含量显著降低(P<0.05);p38MAPK含量升高,但差异不显著(P>0.05)。说明NF-κB信号通路在奶牛腐蹄病促炎因子表达调控中可能起着重要作用,p38MAPK信号通路并不是腐蹄病发生期间诱导炎症反应的主要信号通路。In order to clarify the expression and changes in NF-κB and p38 MAPK signaling pathways in dairy cows with foot rot disease,the role of inflammatory signaling pathway in dairy cow with foot rot disease was discussed.The expression of TNF-α,IL-1β,IL-4,p38 MAPK and NF-κB in serum of rotten hoof dairy cows and healthy dairy cows were detected by enzyme-linked immunosorbent assay(ELISA).The results showed that compared with healthy dairy cows,the contents of NF-κB and IL-1βincreased significantly(P<0.05),IL-4 increased significantly(P<0.01),TNF-αdecreased significantly(P<0.05)and p38 MAPK increased in foot rot disease dairy cows,but the difference was not significant(P>0.05).Therefore,NF-κB signaling pathway might play an important role in the regulation of the expression of proinflammatory cytokines in dairy cow hoof disease,the p38 MAPK signaling pathway is not the main signaling pathway that induces inflammatory response during the occurrence of foot rot.

关 键 词：奶牛 腐蹄病 NF-ΚB信号通路 白细胞介素1Β 白细胞介素4 P38MAPK信号通路 

分 类 号：S855[农业科学—临床兽医学] Q78[农业科学—兽医学]