水稻弯孢叶斑病病原鉴定及其生物学特性  

Identification and characterization of causal organism of rice Curvularia leaf spot disease

作　　者：徐辉[1] 侯冕 檀根甲[1] 刘道贵 吴彩铃 徐劲峰 XU Hui;HOU Mian;TAN Genjia;LIU Daogui;WU Cailing;XU Jinfeng(Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes,School of Plant Protection,Anhui Agricultural University,Hefei 230036;Agricultural Technology Extension Center of Guichi District,Guichi 247110;Plant Quarantine and Protection Station of Qianshan City,Qianshan 246300)

机构地区：[1]植物病虫害生物学与绿色防控安徽普通高校重点实验室,安徽农业大学植保学院,合肥230036 [2]贵池区农技推广中心,贵池247110 [3]潜山市植保站,潜山246300

出　　处：《安徽农业大学学报》2020年第1期129-134,共6页Journal of Anhui Agricultural University

基　　金：安徽省重点研发项目(1804a07020139);国家重点研发项目(2016YFD0200806)共同资助。

摘　　要：为了明确安徽省水稻叶片上发生的一种新病害,从潜山稻田采集病样,利用形态学和分子生物学方法对水稻弯孢叶斑病的病原进行了鉴定,测定了其生物学特性。结果表明,该病原菌为新月弯孢霉Curvularia lunata,最适于病原菌菌丝生长和产孢的温度为30℃,最适于孢子萌发的温度为25~30℃,致死温度为53℃10 min,最适于菌丝生长、孢子萌发和产孢的pH为6~8,光照对其无影响,最适碳源为蔗糖,最适氮源为铵态氮。In order to identify the pathogen of a new leaf spot disease on rice and to study the biological characteristics, the samples of leaf spot disease on rice were collected from a rice field in Qianshan,Anhui. The results showed that the pathogen was identified morphologically and molecularly as Curvularia lunata., the pathogenicity test on leaves with needle-punching method matched the symptoms described above and re-isolated the fungus confirming the Koch’s postulate. The optimum temperature for mycelia growth and spore germination was 30℃ and 25-30℃ respectively. The lethal temperature for mycelia growth was 53℃ for 10 min;The suitable pH was 6-8. The mycelium of Curvularia lunata grew well on the medium with sucrose as the carbon sources. Ammonium nitrogen was the best nitrogen source. Light had little effect on the pathogen.

关 键 词：水稻 新月弯孢霉 病原鉴定 生物学特性 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]