内蒙古地区蒙汉族早产儿支气管肺发育不良与SP-B内含子4基因多态性的相关性研究  

The relationship between bronchopulmonary dysplasia and SP-B intron 4 gene polymorphism in Mongolian and Han newborns in Inner Mongolia

作　　者：霍梦月 梅花[1] 刘春枝[1] 张钰恒 新春 贾昕[1] 闫菲[1] 张艳波[1] 胡亚楠[1] Huo Mengyue;Mei Hua;Liu Chunzhi;Zhang Yuheng;Xin Chun;Jia Xin;Yan Fei;Zhang Yanbo;Hu Yanan(Department of Neonatology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院新生儿科,呼和浩特010050

出　　处：《中华新生儿科杂志（中英文）》2020年第2期87-91,共5页Chinese Journal of Neonatology

基　　金：内蒙古自治区自然科学基金项目(2015MS(LH)0810、2016MS(LH)0804);内蒙古医科大学附属医院重大科研项目(NYFY ZD008)。

摘　　要：目的探讨肺表面活性蛋白B(surfactant proteins-B,SP-B)内含子4基因多态性与早产儿支气管肺发育不良(bronchopulmonary dysplasia,BPD)的相关性。方法选取2016年1月至2019年1月内蒙古医科大学附属医院新生儿科诊断为BPD的早产儿为病例组,同期同民族非BPD早产儿为对照组,进行前瞻性研究,采用聚合酶链反应扩增基因分析技术分析SP-B内含子4基因型及等位基因分布情况。结果共纳入BPD患儿74例,其中蒙古族30例,汉族44例;对照组134例,其中蒙古族56例,汉族78例。不论蒙古族或汉族患儿,SP-B内含子4基因均可检出野生型和变异型(包括插入型及缺失型)2种基因型。蒙古族BPD患儿变异型基因型频率及等位基因频率与对照组相比[36.7%(11/30)比19.6%(11/56),21.7%(13/60)比12.5%(14/112)]差异无统计学意义(P>0.05);汉族BPD患儿变异型基因型频率及等位基因频率均高于对照组[31.8%(14/44)比12.8%(10/78),20.5%(18/88)比7.1%(11/156)],差异有统计学意义(P<0.05)。结论SP-B内含子4基因变异可能与内蒙古地区汉族BPD患儿遗传易感性相关。Objective To study the relationship between pulmonary surfactant protein B(SP-B)intron 4 gene polymorphism and bronchopulmonary dysplasia(BPD)in premature infants.Method From January 2016 to January 2019,premature infants diagnosed with BPD in our hospital were selected as the BPD group,and non-BPD premature infants of the same ethnic group were selected as the control group.The genotype and allele distribution of SP-B intron 4 were analyzed using polymerase chain reaction(PCR)method.Result A total of 74 infants with BPD were included,including 30 Mongolian infants and 44 Han infants.A total of 134 cases were in the control group,including 56 Mongolian infants and 78 Han infants.Wild type and variant type(including insertion and deletion)could be detected in SP-B intron 4 gene in both Mongolian and Han infants.The frequencies of wild and variant genotypes and alleles in Mongolian BPD infants were similar with the control group[36.7%(11/30)vs.19.6%(11/56),21.7%(13/60)vs.12.5%(14/112)](P>0.05).The frequencies of wild and variant genotypes and alleles in Han infants with BPD were significantly different from the control group[31.8%(14/44)vs.12.8%(10/78),20.5%(18/88)vs.7.1%(11/156)](P<0.05).Conclusion The variation of intron 4 gene in SP-B may be related with the genetic susceptibility of Han infants with BPD in Inner Mongolia.

关 键 词：支气管肺发育不良 肺表面活性物质相关蛋白质B 内含子4 基因型 

分 类 号：R722[医药卫生—儿科]