哮喘-慢性阻塞性肺疾病重叠综合征患者呼出气冷凝集液中TNF-α、IL-8、IL-10的检测水平及意义  

Detection levels and significances of TNF-α,IL-8,and IL-10 in exhaled breath condensate of patients with asthma-chronic obstructive pulmonary disease overlap syndrome

作　　者：陈锋[1] 李群[1] 吴迪[1] 严方涛 苏娴[1] 王月宾[1] 梁秋萍[1] Chen Feng;Li Qun;Wu Di;Yan Fangtao;Su Xian;Wang Yuebin;Liang Qiuping(Department of Respiratory and Critical Care Medicine,Chetigdu Third People's Hospital Chengdu Institute of Respiratory Health,Chengdu 6100311 China)

机构地区：[1]成都市第三人民医院呼吸与危重症医学科成都市呼吸健康研究所,610031

出　　处：《国际呼吸杂志》2020年第8期566-571,共6页International Journal of Respiration

基　　金：2016年成都市卫计委科研课题(2016026)。

摘　　要：目的检测哮喘-慢性阻塞性肺疾病重叠综合征(ACOS)患者呼出气冷凝集液(EBC)中肿瘤坏死因子α(TNF-α)、白细胞介素8(IL-8)、IL-10水平,并分析其与该疾病的关系.方法选取2016年2月至2019年3月成都市第三人民医院收治的ACOS患者105例,单纯哮喘患者109例(哮喘组),单纯COPD患者104例(COPD组),另选取同期体检的正常人100例(健康对照组).酶联免疫吸附法检测EBC中TNF-α、IL-8、IL-10水平,自动血液仪检测外周血嗜酸粒细胞比例,利用肺功能仪检测所有受试者第1秒用力呼气容积(FEV1)、用力肺活量(FVC).结果ACOS组、哮喘组、COPD组患者血清IgE水平、外周血嗜酸粒细胞显著高于健康对照组(t=13.594、23.188、3.595;20.886、26.966、20.036,P值均<0.05).ACOS组EBC中TNF-α、IL-8水平显著高于COPD组、哮喘组、健康对照组(t=11.999、13.157、26.807;6.961、7.741、32.496,P值均<0.05),IL-10水平、FEV1%pred显著低于哮喘组、COPD组、健康对照组(t=7.566、9.568、40.129;t=11.539、14.339、28.837,P值均<0.05),FEV1/FVC值显著低于健康对照组(t=16.756,P<0.05);COPD组、哮喘组EBC中TNF-α水平、IL-8水平显著高于健康对照组(t=14.892、14.057;25.544、25.146,P值均<0.05),IL-10水平、FEV1%pred、FEV1/FVC值显著低于健康对照组(t=32.562、31.045;t=17.372、14.939;13.752、16.341,P值均<0.05);哮喘组和COPD组TNF-α、IL-8、IL-10水平、FEV1%pred、FEV1/FVC值比较差异无统计学意义(t=1.015、0.697、1.909、2.658、2.457,P值均>0.05).Pearson检验结果显示,ACOS患者EBC中TNF-α、IL-8水平与FEV1%pred、FEV1/FVC呈负相关(r=-0.463、-0.504;-0.447、-0.476,P值均<0.05);IL-10水平与FEV1%pred、FEV1/FVC呈正相关(r=0.429、0.474,P值均<0.05);Logistic回归分析结果显示,血清IgE水平升高、EBC中TNF-α、IL-8水平升高是ACOS的危险因素(P<0.05),EBC中IL-10水平升高是ACOS的保护因素(P<0.05).结论EBC中TNF-α、IL-8、IL-10水平可能具有潜在的鉴别ACOS生物标志价值.Objective To detect the levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-8,and IL-10 in exhaled breath condensate(EBC)of patients with asthma-chronic obstructive pulmonary disease overlap syndrome(ACOS),and to analyze their relationships with the disease.Methods 105 patients with ACOS,109 patients with asthma,and 104 patients with COPD were selected in Chengdu Third People's Hospital from February 2016 to March 2019,and 100 normal persons were selected from the same period.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of TNF-α,IL-8 and IL-10 in EBC,the ratio of eosinophils in peripheral blood was detected by automatic hematology analyzer,lung function instrument was used to detect forced expiratory volume in one second(FEV1)and forced vital capacity(FVC)in all subjects.Results Serum IgE level and peripheral blood eosinophils in ACOS group,asthma group,and COPD group were significantly higher than those of in healthy control group(t=13.594,23.188,3.595;20.886,26.966,20.036,all P<0.05).The levels of TNF-α and IL-8 in EBC of ACOS group were significantly higher than those of in COPD group,asthma group and healthy control group(t=11.999,13.157,26.807;6.961,7.741,32.496,all P<0.05),the level of IL-10,forced expiratory volume in one second as a percentage of expected value(FEV1%pred)were significantly lower than those of in asthma group,COPD group,and healthy control group(t=7.566,9.568,40.129;t=11.539,14.339,28.837,all P<0.05),and FEV1/FVC value was significantly lower than healthy control group(t=16.756,P<0.05).The levels of TNF-α and IL-8 in EBC of COPD group and asthma group were significantly higher than those of healthy control group(t=14.892,14.057;25.544,25.146,all P<0.05),the levels of IL-10,FEV1%pred,and FEV1/FVC value of COPD group and asthma group were significantly lower than those of healthy control group(t=32.562,31.045;t=17.372,14.939;13.752,16.341,all P<0.05).There were no significant differences in TNF-α level,IL-8 level,IL-10 level,FEV1%pred and FEV1/FVC value be

关 键 词：肿瘤坏死因子α 白细胞介素8 白细胞介素10 哮喘-慢性阻塞性肺疾病重叠综合征 呼出气冷凝集液 

分 类 号：R563[医药卫生—呼吸系统]