线粒体解偶联蛋白1基因缺陷小鼠淋巴细胞的频率及活性变化  

Variations of frequencies and activities of lymphocytes in the mitochondrial uncoupling protein 1 deficient mice

作　　者：温建强 蔺志杰 张瑜[1] 路国涛[2] 龚卫娟[1,2] WEN Jianqiang;LIN Zhijie;ZHANG Yu;LU Guotao;GONG Weijuan(Teaching and Research Section of Immunology,Medical College of Yangzhou University,Yangzhou,Jiangsu,225001;Department of Gastroenterology,The Affiliated Hospital of Yangzhou University,Yangzhou,Jiangsu,225001)

机构地区：[1]扬州大学医学院免疫学教研室,江苏扬州225001 [2]扬州大学附属医院消化科,江苏扬州225001

出　　处：《实用临床医药杂志》2020年第4期16-20,25,共6页Journal of Clinical Medicine in Practice

基　　金：国家自然科学基金资助项目(81671547,81873866,81873867);江苏省研究生学术创新计划项目(KYCX19_2122)。

摘　　要：目的探讨解偶联蛋白1(UCP1)基因缺陷小鼠体内淋巴细胞的频率及其功能变化。方法利用流式细胞术检测UCP1^-/-小鼠和对照组小鼠脾脏、外周血、胸腺或骨髓CD4^+T细胞、CD8^+T细胞、自然杀伤细胞(NK细胞)和B淋巴细胞的频率;并利用胞内染色法,检测CD4^+CD25^+Foxp3+调节性T细胞的频率以及CD4^+和CD8^+产生干扰素-γ(IFN-γ)的活性。结果生理状态下UCP1^-/-小鼠和对照小鼠相比,胸腺T细胞、NK细胞及骨髓B细胞的频率无显著差异;UCP1^-/-小鼠脾脏总CD8^+T细胞频率、活化性CD8+T细胞(CD8^+NKG2D^+、CD8^+CD44^+CD62L-)和效应性CD8^+T细胞(CD8^+CD44^+KLRG1+)频率下降,且IFN-γ分泌减少;脾脏和外周血CD4^+T细胞的频率及其活性,NK细胞和B细胞的频率均无显著变化。结论UCP1缺陷对淋巴细胞的发育无影响,但下调CD8^+T细胞的活性。Objective To analyze variations of frequencies and biologic activities of lymphocytes in the uncoupling protein 1 deficient(UCP1^-/-)mice.Methods The frequencies of CD4^+T,CD8^+T,natural killer cell(NK cell),and B lymphocyte in spleen,peripheral blood,thymus or bone marrow were measured both from the wild-type(WT)and knock-out(KO)mice by flow cytometry.The CD4^+CD25^+Foxp3^+cell frequencies and interferon-γ(IFN-γ)production of CD4^+T or CD8^+T cells were analyzed by the flow-cytometric intracellular staining.Results Physiologically,there were no significant differences of T and NK cell frequencies in thymus and B cell frequency in bone marrow between two kinds of mice.Compared with WT mice,frequencies of total CD8^+T cells,activated CD8+T cells(CD8+NKG2D+and CD8^+CD44^+CD62L-)and effector CD8+T cells(CD8^+CD44^+KLRG1^+)decreased in KO mice.IFN-γproduction by UCP1^-/-CD8+T cells was also lower than that of CD8^+T cells in WT mice.No significant variations of CD4^+T cell frequency and activity in spleen and blood of the KO mice were observed.Neither NK nor B cell frequencies in spleen and blood were different between two kinds of mice.Conclusion The UCP1 deficiency may exert no effects on the development of lymphocytes,but can down regulate biologic activities of CD8^+T cells.

关 键 词：解偶联蛋白1 淋巴细胞 脾脏 外周血 胸腺 骨髓 活性 

分 类 号：R392.33[医药卫生—免疫学]